B6.129X1(129S4)-Il6tm1.1Jho/Cnbc
| Status | Available to order |
| EMMA ID | EM:05332 |
| International strain name | B6.129X1(129S4)-Il6tm1.1Jho/Cnbc |
| Alternative name | Interleukin-6 floxed mice |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Il6tm1.1Jho, |
| Gene/Transgene symbol | Il6 |
Information from provider
| Provider | Juan Hidalgo |
| Provider affiliation | Biología Celular, Fisiología e Inmunología, Universidad Autónoma de Barcelona |
| Genetic information | Floxed mouse line for interleukin 6 (Il6). To this end, exon 2 from Il6 gene has been flanked with loxP sites. |
| Phenotypic information | With this number of backcrosses (ca. 5) the floxed mice show some phenotype, since the floxed Il6 gene is expressed less than in normal wild-type mice upon endotoxin administration. We have already produced some conditional KO mice with several cre-expressing mouse lines and there is a clear difference between floxed and KO mice regarding Il6 levels. |
| Breeding history | Neo cassette was removed by crossing with flp-expressing mice once one of the chimeras was found to have germ line transmission upon crossing with C57BL/6. The flp gene was removed upon crossing with C57BL/6. Finally, offspring mice positive for the floxed gene were crossed with each other and a homozygous line of these floxed mice is maintained that way in the general animal services of this university. We have started to further backcross this line with C57BL/6 in the Animal Physiology unit; in total we are about in the fifth generation. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6JOlaHsd, wild-type C57BL/6JOlaHsd |
| Stage of embryos | 2-cell |
Literature references
- Astrocyte-specific deficiency of interleukin-6 and its receptor reveal specific roles in survival, body weight and behavior.;Quintana Albert, Erta Maria, Ferrer Beatriz, Comes Gemma, Giralt Mercedes, Hidalgo Juan, ;2013;Brain, behavior, and immunity;27;162-73; 23085146
- Identification of liver cancer progenitors whose malignant progression depends on autocrine IL-6 signaling.;He Guobin, Dhar Debanjan, Nakagawa Hayato, Font-Burgada Joan, Ogata Hisanobu, Jiang Yuhong, Shalapour Shabnam, Seki Ekihiro, Yost Shawn E, Jepsen Kristen, Frazer Kelly A, Harismendy Olivier, Hatziapostolou Maria, Iliopoulos Dimitrios, Suetsugu Atsushi, Hoffman Robert M, Tateishi Ryosuke, Koike Kazuhiko, Karin Michael, ;2013;Cell;155;384-96; 24120137
- MHC class II-dependent B cell APC function is required for induction of CNS autoimmunity independent of myelin-specific antibodies.;Molnarfi Nicolas, Schulze-Topphoff Ulf, Weber Martin S, Patarroyo Juan C, Prod'homme Thomas, Varrin-Doyer Michel, Shetty Aparna, Linington Christopher, Slavin Anthony J, Hidalgo Juan, Jenne Dieter E, Wekerle Hartmut, Sobel Raymond A, Bernard Claude C A, Shlomchik Mark J, Zamvil Scott S, ;2013;The Journal of experimental medicine;210;2921-37; 24323356