B6;Cg-Nrp1tm1.1Cruh/H

Status

Available to order

EMMA IDEM:11987
International strain nameB6;Cg-Nrp1tm1.1Cruh/H
Alternative nameNrp1cyto
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolNrp1tm1.1Cruh,
Gene/Transgene symbolNrp1

Information from provider

ProviderChristiana Ruhrberg
Provider affiliationUCL Institute of Ophthalmology, University College London
Genetic informationA 4 base-pair insertion (AATT) into the Nrp1 last exon after the transmembrane domain coding sequence, shifting the reading frame and preventing translation of the cytoplasmic domain of Nrp1.
Phenotypic informationHomozygous:
The analysis of pre- and perinatal vascular development revealed that vasculogenesis and angiogenesis proceed normally in these mutants, demonstrating that the Nrp1 membrane-anchored extracellular domain is sufficient for vessel growth. By contrast, the cytoplasmic domain is required for normal arteriovenous patterning, because arteries and veins crossed each other at an abnormally high frequency in the Nrp1-cyto delta/delta retina. At crossing sites, the artery was positioned anteriorly to the vein, and both vessels were embedded in a shared collagen sleeve.

Heterozygous:
--
References
  • The cytoplasmic domain of neuropilin 1 is dispensable for angiogenesis, but promotes the spatial separation of retinal arteries and veins.;Fantin Alessandro, Schwarz Quenten, Davidson Kathryn, Normando Eduardo M, Denti Laura, Ruhrberg Christiana, ;2011;Development (Cambridge, England);138;4185-91; 21852397
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI allele-associated human disease models

Literature references

  • The cytoplasmic domain of neuropilin 1 is dispensable for angiogenesis, but promotes the spatial separation of retinal arteries and veins.;Fantin Alessandro, Schwarz Quenten, Davidson Kathryn, Normando Eduardo M, Denti Laura, Ruhrberg Christiana, ;2011;Development (Cambridge, England);138;4185-91; 21852397

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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Practical information

Example health report
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