Selected reading cryobiology

INFRAFRONTIER has collected publications covering different aspects of cryopreservation techniques. The EMMA partners find these articles and reviews useful for scientists who want to apply archiving techniques. The lists are not exhaustive.

Cryopreservation general

Key references

  • Abbott A. Geneticists prepare for deluge of mutant mice. Nature 2004; 432: 541. PMID: 15577874

  • Bredkjaer HE, Grudzinskas JG. Cryobiology in human assisted reproductive technology. Would Hippocrates approve? Early Pregnancy 2001; 5: 211-3. PMID: 11753534

  • Caperton L, Murphey P, Yamazaki Y, McMahan CA, Walter CA, Yanagimachi R, et al. Assisted reproductive technologies do not alter mutation frequency or spectrum. Proc Natl Acad Sci U S A 2007; 104: 5085-90. PMID: 17360354

  • Crabbe JC, Schneider U, Hall JW, Mazur P. Invited commentary: cryopreservation as a tool for the study of selectively bred lines in rodent behavioral genetics. Behav Genet 1993; 23: 307-12. PMID: 8240209

  • Fahning ML, Garcia MA. Status of cryopreservation of embryos from domestic animals. Cryobiology 1992; 29: 1-18. PMID: 1606826

  • Fray, M.D. 2009. Biological methods for archiving and maintaining mutant laboratory mice. Part I: conserving mutant strains. Methods Mol Biol 561:301-319. PMID: 19504080

  • Fray, M.D. 2009. Biological methods for archiving and maintaining mutant laboratory mice. Part II: recovery and distribution of conserved mutant strains. Methods Mol Biol 561:321-332. PMID: 19504081

  • Fray, M.D., A.R. Pickard, M. Harrison, and M.T. Cheeseman. 2008. Upgrading mouse health and welfare: direct benefits of a large-scale rederivation programme. Lab Anim 42:127-139. PMID: 18435871

  • Glenister PH, Thornton CE. Cryoconservation--archiving for the future. Mamm Genome 2000; 11: 565-71. PMID: 10886024

  • Glenister PH, Whittingham DG, Wood MJ. Genome cryopreservation: a valuable contribution to mammalian genetic research. Genet Res 1990; 56: 253-8. PMID: 2272516

  • Guan M, Marschall S, Raspa M, Pickard AR and Fray MD. Overview of new developments in and the future of cryopreservation in the laboratory mouse. Mamm Genome 2012; 23: 572-9. PMID: 22936001

  • Kasai M. Cryopreservation of mammalian embryos. Mol Biotechnol 1997; 7: 173-9. PMID: 9219232

  • Landel CP. Archiving mouse strains by cryopreservation. Lab Anim (NY) 2005; 34: 50-7. PMID: 15806091

  • Liebermann J, Nawroth F, Isachenko V, Isachenko E, Rahimi G, Tucker MJ. Potential importance of vitrification in reproductive medicine. Biol Reprod 2002; 67: 1671-80. PMID: 12444040

  • Marschall S, Hrabe de Angelis M. In vitro fertilization/cryopreservation. Methods Mol Biol 2003; 209: 35-50. PMID: 12357961

  • Mazur P, Leibo SP, Seidel GE, Jr. Cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions. Biol Reprod 2008; 78: 2-12. PMID: 17901073

  • Pomeroy KO. Cryopreservation of transgenic mice. Genet Anal Tech Appl 1991; 8: 95-101. PMID: 2064824 

  • Scavizzi F, Ryder E, Newman S, Raspa M, Gleeson D, Wardle-Jones H et al. Blastocyst genotyping for quality control of mouse mutant archives: an ethical and economical approach. Transgenic Res 2015; 24(5):921-7. PMID: 26178246

  • Schneider U. Cryobiological principles of embryo freezing. J In Vitro Fert Embryo Transf 1986; 3: 3-9. PMID: 3958565

  • Shaw JM, Kasai M. Embryo cryopreservation for transgenic mouse lines. Methods Mol Biol 2001; 158: 397-419. PMID: 11236670

  • Shaw JM, Nakagata N. Cryopreservation of transgenic mouse lines. Methods Mol Biol 2002; 180: 207-28. PMID: 11873651

  • Woods EJ, Benson JD, Agca Y, Critser JK. Fundamental cryobiology of reproductive cells and tissues. Cryobiology 2004; 48: 146-56. PMID: 15094091

Freezing of mouse embryos

Key references

  • Byers SL, Payson SJ, Taft RA. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 2006; 65: 1716-26. PMID: 16271754

  • Renard JP, Babinet C. High survival of mouse embryos after rapid freezing and thawing inside plastic straws with 1-2 propanediol as cryoprotectant. J Exp Zool 1984; 230: 443-8. PMID: 6747572

  • Richa J. Cryopreservation of mouse embryos. Methods Mol Biol 2000; 135: 77-82. PMID: 10791306

  • Tao J, Tamis R, Fink K. Cryopreservation of mouse embryos at morula/compact stage. J Assist Reprod Genet 2001; 18: 235-43. PMID: 11432117

  • Whittingham DG. Survival of mouse embryos after freezing and thawing. Nature 1971; 233: 125-6. PMID: 12058753

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Freezing of mouse embryos by vitrification

Key references 

  • Kasai M. Cryopreservation of mammalian embryos. Vitrification. Methods Mol Biol 1995; 38: 211-9. PMID: 7647860

  • Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990; 89: 91-7. PMID: 2374136

  • Mazur P. Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos. Cell Biophys 1990; 17: 53-92. PMID: 1704816

  • Nakao K, Nakagata N, Katsuki M. Simple and efficient vitrification procedure for cryopreservation of mouse embryos. Exp Anim 1997; 46: 231-4. PMID: 9250485

  • Walker DL, Tummon IS, Hammitt DG, Session DR, Dumesic DA, Thornhill AR. Vitrification versus programmable rate freezing of late stage murine embryos: a randomized comparison prior to application in clinical IVF. Reprod Biomed Online 2004; 8: 558-68. PMID: 15151720

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Freezing of mouse spermatozoa

Key references

  • Landel CP. Archiving mouse strains by cryopreservation. Lab Anim (NY) 2005; 34: 50-7. PMID: 15806091

  • Marschall, S., A. Boersma, and M.H. de Angelis. 2009. Sperm cryopreservation and in vitro fertilization. Methods Mol Biol 530:407-420. PMID: 19266334

  • Marschall S, Huffstadt U, Balling R, Hrabe de Angelis M. Reliable recovery of inbred mouse lines using cryopreserved spermatozoa. Mamm Genome 1999; 10: 773-6. PMID: 10430662

  • Nakagata N. Cryopreservation of mouse spermatozoa. Mamm Genome 2000; 11: 572-6. PMID: 10886025

  • Ogonuki, N., K. Mochida, H. Miki, K. Inoue, M. Fray, T. Iwaki, K. Moriwaki, Y. Obata, K. Morozumi, R. Yanagimachi, and A. Ogura. 2006. Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring. Proc Natl Acad Sci U S A 103:13098-13103. PMID: 16920794

  • Ostermeier GC, Wiles MV, Farley JS, Taft RA. Conserving, distributing and managing genetically modified mouse lines by sperm cryopreservation. PLoS ONE 2008; 3: e2792. PMID: 18665210 

  • Raspa M, Fray M, Paoletti R, Montoliu R, Giuliani A, EMMA/Infrafrontier Technical Working Group, Scavizzi F. Theriogenology 2017; 107: 41-49. Long term maintenance of frozen mouse spermatozoa at -80 °C PMID: 29128700 

  • Raspa M, Guan M, Paoletti R, Montoliu L, e, Ayadi A, Marschall S, et al. Dry ice is a reliable substrate for the distribution of frozen mouse spermatozoa: A multi-centric study. Theriogenology 2017; 96: 49-57. PMID: 285328

  • Sztein JM, Farley JS, Mobraaten LE. In vitro fertilization with cryopreserved inbred mouse sperm. Biol Reprod 2000; 63: 1774-80. PMID: 11090448

  • Takeo T and Nakagata N. Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin. Biol Reprod 2011; 85: 1066-72. PMID: 21778138

  • Thornton CE, Brown SD, Glenister PH. Large numbers of mice established by in vitro fertilization with cryopreserved spermatozoa: implications and applications for genetic resource banks, mutagenesis screens, and mouse backcrosses. Mamm Genome 1999; 10: 987-92. PMID: 10501968

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Freezing of oocytes

Key references

  • Bouquet M, Selva J, Auroux M. Effects of cooling and equilibration in DMSO, and cryopreservation of mouse oocytes, on the rates of in vitro fertilization, development, and chromosomal abnormalities. Mol Reprod Dev 1995; 40: 110-5. PMID: 7702864

  • Eroglu A, Bailey SE, Toner M, Toth TL. Successful Cryopreservation of Mouse Oocytes by Using Low Concentrations of Trehalose and Dimethylsulfoxide. Biol Reprod 2008. PMID: 18815355

  • Ko CS, Ding DC, Chu TW, Chu YN, Chen IC, Chen WH, et al. Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods. Anim Reprod Sci 2008; 105: 272-82. PMID: 17434695

  • Nakagata N, Takeo T, Fukumoto K, Kondo T, Haruguchi Y, Takeshita Y, Nakamuta Y, Matsunaga H, Tsuchiyama S, Ishizuka Y, Araki K. Applications of cryopreserved unfertilized mouse oocytes for in vitro fertilization. Cryobiology 2013; 67(2): 188-92. PMID: 23846105

  • Rayos AA, Takahashi Y, Hishinuma M, Kanagawa H. Quick freezing of unfertilized mouse oocytes using ethylene glycol with sucrose or trehalose. J Reprod Fertil 1994; 100: 123-9. PMID: 8182579

  • Revel A, Moshe N, Helman A, Safran A, Simon A, Koler M. Mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation. Hum Reprod 2004; 19: 666-9. PMID: 14998968

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Freezing of ovaries

Key references

  • Liu HC, He Z, Rosenwaks Z. Mouse ovarian tissue cryopreservation has only a minor effect on in vitro follicular maturation and gene expression. J Assist Reprod Genet 2003; 20: 421-31. PMID: 14649382

  • Migishima F, Suzuki-Migishima R, Song SY, Kuramochi T, Azuma S, Nishijima M, et al. Successful cryopreservation of mouse ovaries by vitrification. Biol Reprod 2003; 68: 881-7. PMID: 12604638

  • Shaw JM, Trounson AO. Ovarian tissue transplantation and cryopreservation. Application to maintenance and recovery of transgenic and inbred mouse lines. Methods Mol Biol 2002; 180: 229-51. PMID: 11873652

  • Sztein J, Sweet H, Farley J, Mobraaten L. Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. Biol Reprod 1998; 58: 1071-4. PMID: 9546742

  • Tokieda Y, Ishiwata I, Segino M, Ishikawa H, Sato K. Establishment of a novel method for cryopreservation and thawing of the mouse ovary. Hum Cell 2002; 15: 230-7. PMID: 12889859

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