The European Mouse Mutant Archive – EMMA strains are cryopreserved as frozen embryos and/or sperm. Highly demanded EMMA strains are maintained on shelf to facilitate fast delivery to the users. For all other EMMA strains a rederivation service is provided. The kind of material that is in stock can be viewed on the list of available EMMA strains.
|Kind of material||Quantity||Delivery time 1||Recommended EMMA service fee 2,3|
|Frozen embryos||40-50 embryos||4 weeks||€1600|
|Frozen sperm 4||2 straws/vials||4 weeks||€1600|
|Live mice on shelf||up to 3 heterozygous|
|4 weeks 6||€3200|
Rederivation of mice
|up to 3 heterozygous|
|4-6 months 6||€3200|
Allele conversion service
|up to 3 heterozygous|
1) From the time when all paperwork is in place until when the material is ready for shipping at EMMA nodes.
2) In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).
3) For strains generated by use of the CRISPR technology, users also have to pay a license surcharge.
4) Only recommended for users who have IVF capability.
5) In general, up to 3 heterozygous animals, males or females, will be shipped. Wildtype littermates could be also available upon request.
6) For special requirements (e.g. certain age, sex and/or number of live mice are required), longer service time may be expected.
Recommended EMMA service fees
The recommended EMMA service fees enable the INFRAFRONTIER partners to recover their costs and ensure that INFRAFRONTIER maintains a sustainable archiving and distribution service.
In addition to these recommended EMMA service fees users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable). For strains generated by use of the CRISPR technology, users also have to pay a license surcharge. In case of cancellations of service requests, EMMA reserves the right to charge users for incurred costs. As EMMA is not a legal entity, the individual EMMA nodes will invoice users separately.
Kind of material and delivery times
Due to its methodological simplicity and reduced costs, the default archival material is frozen sperm obtained from mice that are heterozygous for the mutation(s). Please be aware that after rederivation from frozen sperm the mutant mice obtained will not be homozygous and the background may slightly differ from the original one depending on the oocytes strain used for the recovery. Frozen material and live mice of strains that are maintained on shelf can be shipped within four weeks. Should specific age, sex, or number of live mice be enquired for, it can take longer to meet the users’ requirements. Rederivation of mice from frozen stock usually takes four to six months.
In general, service requests will not be processed until a fully executed MTA has been received (where applicable) and either a billing address or a purchase order number has been provided by the customer. Therefore, please note that the delivery times given above represent the time period between the date when all paperwork is in place and the date of when the material is available for shipping at EMMA nodes.
The frozen material provided will be sufficient for two IVF or embryo transfer sessions. If live mice are ordered, EMMA will ship up to 3 heterozygous animals, males or females (wildtype littermates could be also available upon request).
EMMA does not generally offer breeding services to e.g. generate cohorts of homozygous mutants. However, such a service and fees may be negotiated with the respective EMMA distribution centre.
EMMA screens and distributes mice in accordance with FELASA protocols and guarantees that mice leaving the EMMA institutions are free of pathogens as specified by FELASA.
The quality control provided by INFRAFRONTIER/EMMA for community deposited strains, EUCOMM/KOMP-CSD and CRISPR/Cas9 mice is detailed here. However, specific lines may be mantained at a slightly different quality control level. The quality control level applied to each strain is explained in the automated confirmation e-mail sent to the users upon completion of their request online individually.
Intellectual property rights
Deposited lines are distributed under the EMMA repository conditions. EMMA only acts as distributor and any already existing Material Transfer Agreement will remain in full force and effect.
How to order
Please visit the EMMA strain list, find your strain of interest, open the strain details by using the '+' toggle, then click the 'Order' button. EMMA will contact you within two weeks after receipt of your EMMA Mutant Request Form. For more information about the order process please check the FAQs section.
Strains under development
EMMA has newly submitted strains in various stages of preservation, development and colony establishment. These strains are not yet available for distribution or ordering and thus marked by a red traffic light symbol. However, you are invited to register your interest in any such strains by clicking on the EMMA strain list, finding your strain of interest, opening the strain details by using the '+' toggle, then clicking the 'Register interest' button.
Registrations of interest will be handled on a first-come, first-served basis. This registration of interest is valuable in helping EMMA to anticipate demand and to optimise availability of strains. Users will be notified as soon as the strain becomes available. They will receive an e-mail with a link to a pre-filled online form that they can use to submit their order.
EMMA strains produced from ES cells generated by the International Knockout Mouse Consortium (IKMC) are displayed on the EMMA strain list as soon as germ-line transmission has been achieved and the genotype of the pups has been confirmed. From this stage on scientists can register their interest for the mice on the EMMA strain list as described above.
Genes that have only been assigned for mouse production and phenotyping by the International Mouse Phenotyping Consortium (IMPC) and for which mice have not been produced, yet, it is possible to register interest on the IMPC webpage.
ES cells and targeting vectors can be ordered directly from the IKMC repositories. The European Mouse Mutant Cell Repository (EuMMCR) holds and distributes vectors and mutant ES cell lines produced in the EUCOMM and EUCOMMTools consortia representing over 16.800 different genes.