References

Selected literature illustrating recent advances in disentangling mouse genotype and microbiota contribution to phenotypes

Metabolism

  • Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbiol ecology: human gut microbes associated with obesity. Nature 2006; 444: 1022-3. PMID: 17183309

Behaviour

  • Heijtz RD, Wang S, Anuar F, Qian Y, Björkholm B, Samuelsson A, Hibberd ML, Forssberg H, Pettersson S. Normal gut microbiota modulates brain development and behavior. Proc Natl Acad Sci U S A 2010; 108: 3047-52. PMID: 21282636
  • Mayer EA. Gut feelings: the emerging biology of gut-brain communication. Nat Rev Neurosci 2011; 12: 453-66. PMID: 21750565

Autoimmune diseases and allergy

  • Berer K, Mues M, Koutrolos M, Rasbi ZA, Boziki M, Johner C, Wekerle H, Krishnamoorthy G. Commensal microbiota and myelin autoantigen cooperate to trigger autoimmune demyelination. Nature 2011; 479: 538-41. PMID: 22031325
  • Hadis U, Wahl B, Schulz O, Hardtke-Wolenski M, Schippers A, Wagner N, Müller W, Sparwasser T, Förster R, Pabst O. Intestinal tolerance requires gut homing and expansion of FoxP3+ regulatory T cells in the lamina propria. Immunity 2011; 34: 237-46. PMID: 21333554
  • Wen L, Ley RE, Volchkov PY, Stranges PB, Avanesyan L, Stonebraker AC, Hu C, Wong FS, Szot GL, Bluestone JA, Gordon JI, Chervonsky AV. Innate immunity and intestinal microbiota in the development of Type 1 diabetes. Nature 2008; 455: 1109-13. PMID: 18806780

Infection

  • Kane M, Case LK, Kopaskie K, Kozlova A, MacDearmid C, Chervonsky AV, Golovkina TV. Successful transmission of a retrovirus depends on the commensal microbiota. Science 2011; 334: 245-9. PMID: 21998394
  • Kuss SK, Best GT, Etheredge CA, Pruijssers AJ, Frierson JM, Hooper LV, Dermody TS, Pfeiffer JK. Intestinal microbiota promote enteric virus replication and systemic pathogenesis. Science 2011; 334: 249-52. PMID: 21998395

Technical description on how to raise germ-free mice

  • Giraud A. Axenic mice model. Methods Mol Biol 2008; 415: 321-36. PMID: 18370163

Proxy for axenization: large spectrum antibiotics treatment

  • Rakoff-Nahoum S, Paglino J, Eslami-Varzaneh F, Edberg S, Medzhitov R. Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell 2004; 118: 229-41. PMID: 15260992

This protocol for the treatment of mice with a cocktail of four antibiotics (ampicillin, neomycin, metronidazole and vancomycin) is often used as a pilot study before processing with full axenization.

 

Contacts

For general enquiries on EMMA germ-free service
Dr. Michael Hagn
The European Mouse Mutant Archive
Helmholtz Zentrum München
Institute of Experimental Genetics
Ingolstädter Landstraße 1
85764 Neuherberg Germany
Phone: +49 89 31873628
FAX: +49 89 31873500

 

For scientific enquiries
EMMA Portugal
Instituto Gulbenkian de Ciência
Rua da Quinta Grande 6, Apartado 14
2781-901 Oeiras, Portugal
Phone: +351 21 440 79 08
FAX : + 351 21 440 79 70