Cryopreservation training courses

EMMA has extensive experience in hosting cryocourses. In the last 12 years the EMMA partners CNR, MRC and CNRS have collectively organised a total of 43 training courses which were attended by over 250 participants. The courses cover state of the art techniques in cryobiology and also more general topics such as handling of experimental animals. During the course of the EC funded INFRAFRONTIER-I3 project (2013 - 2016) a total of 12 cryocourses will be offered to the wider European community. The main course held at the CNR Monterotondo is a joint course with faculty of the Jackson Laboratory. As both Jax and EMMA have a strong focus on continuous innovation of used SOPs, the courses will always cover the latest versions of validated and established SOPs. The SOPs are also disseminated via the INFRAFRONTIER website.

CNR Monterotondo course

CNR Monterotondo Workshop on Cryopreservation and Recovery Techniques in the Laboratory Mouse, November 07-10, 2016

 

The Consiglio Nazionale delle Ricerche, European Mouse Mutant Archive (EMMA) Core Structure, Monterotondo Campus and The Jackson Laboratory offer a comprehensive workshop on cryopreservation of mouse embryos, sperm and ovaries. This Workshop is financially supported by the European Union FP7 Capacities Specific Programme, through the INFRAFRONTIER-I3 project.

The workshop is offered to teach methods in cryopreservation for banking of mouse strains used in research, including inbred, transgenic and targeted mutant strains. Several methods of cryopreservation are now available and because no single method is adequate for all the different strains being developed, a variety of methods are taught. The workshop is designed primarily as a "hands-on" laboratory course program in which participants practice and learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa, oocyte and ovaries, as developed and currently applied by the EMMA-INFRAFRONTIER partner repositories and by the Jackson Laboratory.

 

Techniques include:

  • Embryo two step equilibrium freezing in plastic straws.
  • Embryo and oocyte non-equilibrium ultra-rapid cooling or vitrification in straws.
  • Sperm freezing and recovery of frozen sperm by in vitro fertilization.
  • Shipping frozen, unfrozen embryos and spermatozoa

 

In addition, general principles of cryobiology, development of inventory databases for individual programs, and adaptation of long-term storage systems and cryogenic equipment for different situations will be presented and discussed. Surgical and nonsurgical
mouse embryo transfer techniques will be demonstrated, as well as mouse microinsemination (intracytoplasmic sperm injection) and ovary transplantation.

 

Course flyer November 2016

Application form

 

Past Monterotondo cryocourses
  • 16 - 20 November 2015 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 20 - 24 October 2014 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 21 - 25 October 2013 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 22 - 26 October 2012 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 17 - 21 October 2011 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 25 - 29 October 2010 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 23 - 26 November 2009 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 18 - 20 January 2009 CNR-EMMA-INFRAFRONTIER Short ArchiveFrontier Course on Cryopreservation of Mouse Germplasm
  • 27 - 30 October 2008 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 5 - 8 November 2007 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 13 - 16 November 2006 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 7 - 10 Novembeer 2005 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 14 - 19 November 2004 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
  • 9 - 14 November 2003 CNR-The Jackson Lab.-EMMA Monterotondo Course on Cryopreservation of Mouse Germplasm
MRC Harwell course

MRC Harwell cryocourse, 20-23 March 2017

The course is intended to give animal technologists hands-on experience of the murine embryo and spermatozoa freezing techniques routinely used at Harwell. In addition, delegates will gain experience of a simple and robust in vitro fertilization procedure.

  • Get practical experience in embryo and sperm freezing
  • Learn how to set up a robust IVF protocol
  • Hear about optimized transport methods for shipping unfrozen sperm and embryos
  • Discuss crucial issues for your own scientific projects
  • Courses fee are £1000/person, subsidised accommodation can be provided

 

For further information visit the MRC Harwell training course pages or contact FESA@har.mrc.ac.uk

 

Course flyer March 2017

Course flyer September 2016

Course flyer September 2015

Course flyer March 2014

Course flyer September 2013

VetMedUni Vienna course

University of Veterinary Medicine Vienna

Cryo & Embryo Transfer Course, September 5-9, 2016


The Institute of Laboratory Animal Science and Biomodels Austria at the University of Veterinary Medicine Vienna offer a comprehensive course on cryopreservation, embryo transfer and other methods of assisted reproduction in mice. The course is intended to give technicians and scientists state-of-the-art background knowledge and hands-on training in the methods routinely used at the Vetmeduni Vienna.


The Course will include:
• Theory Lectures (can be booked separately):
Mouse Anatomy and Biology of Reproduction, Introduction to Embryo Transfer, Anesthesia & Analgesia, Introduction to Sperm Freezing, In Vitro Fertilisation and Embryo Freezing methods, The European Mouse Mutant Archive, Worldwide shipping of mice and of cooled / frozen material


• Hands-on Practical Training:
Preparation of Media and Capillaries, Sperm Collection and Cryopreservation, Superovulation, Oviduct and Uterus Flushing, Handling and Culture of Preimplantation Embryos, In vitro Fertilisation, Controlled Freezing of 2-cell embryos, Vasectomy, Plug Check of Recipients, Surgical Oviduct and Uterus Embryo Transfer


• Demonstrations:
Percutaneous and Microsurgical Collection of Spermatozoa from Live Mice, Ovary Transplantation; Non-surgical Embryo Transfer


• Enrollment and course fees: The course is financially supported by the EC FP7 Capacities Specific Program through the INFRAFRONTIER-I3 project. Enrollment is limited to 6 participants for Hands-on Practical Training and to 60 participants for the One Day Theory Lectures. Early application is advised.


European Union's and Associated Countries' nationals:
Academic or Non-Profit Institutions: Euro 500.00
Other Institutions: Euro 900.00


Other Countries' nationals:
Academic or Non-Profit Institutions: Euro 900.00
Other Institutions: Euro 1,200.00


Theory only: Euro 150.00

 

*Fees do not include participant's travel and lodging.

 

For more information and booking please go to:

http://www.vetmeduni.ac.at/de/labortierkunde/kurse/cryo-embryo-transfer-course/

 

Course flyer September 2016

INFRAFRONTIER Cryopreservation Workshop at the 13th ISTT meeting

A Cryopreservation Workshop will be organized in the framework of the TT2016 conference in Prague.
The workshop is lead by Martin Fray and his team (MLC, MRC Harwell)

13th Transgenic Technology Meeting (TT2016)
Mouse Cryopreservation Workshop
16th – 18th March 2016 (Wednesday – Friday)

This 3-day course is intended to give animal technologists hands-on experience of the murine embryo and spermatozoa freezing techniques routinely used at archiving nodes serving the European Mouse Mutant Archive (EMMA). In addition, delegates will gain experience of a simple in vitro fertilization procedure.

Please find more information for the workshop at this link
http://www.transtechsociety.org/tt2016/scientific-programme/workshops/mouse-cryopreservation-workshop/

Applicants need to be registered at the TT2016 conference. Participation on the course is subject to a selection process. Applications must be accompanied by a short CV, letter of motivation, and a short letter of support.