Model development training courses

Partners of the INFRAFRONTIER Research Infrastructure offer unique training opportunities in mouse model development.

 

Upcoming Courses

Practical Training in transgenic technology, 16-18 October 2018, MRC Harwell, UK

The recent emergence of new gene editing techniques such as CRISPR/Cas9 and others has allowed researchers to quickly produce very specific mouse mutants on highly defined genetic backgrounds.

For newcomers to the transgenic field or those looking to brush up their skills, MRC Harwell is offering a practical training course in modern transgenics. Participants will have hands on experience in a technique that will enable them to generate their own mutant mouse models using the CRISPR/Cas9 system.

The course will teach the use of a cytoplasmic injection technique to generate on-target founder mice for both deletion and point mutation projects. This technique is relatively simple to learn when compared with the time required to become competent with pronuclear injections. Participants will also have one-to-one training in embryo harvesting, sorting, set up and operation of their microinjection rig and introducing CRISPR/Cas9 reagents to the embryo using a cytoplasmic injection technique.

In addition, there will be a demonstration of surgical embryo transfer using a surgical assistant to help maintain the sterile condition. Participants will have the opportunity to meet and discuss specific issues with experts in IVF technology, CRISPR/Cas9 design or mouse colony management - depending on the specific needs of the participating researchers.

 

The course will be running from 16 to 18 October 2018. Click here to get the full agenda.

For more details or to register your interest: Please got to the MRC website or send an e-mail to mlc-training@har.mrc.ac.uk

In vivo CRISPR/Cas9 genome editing, 22-23 November 2018, Strasbourg, France

This training aims to provide a general framework to get scientists started using CRISPR-Cas9 for in vivo gene editing in rodents: principles, rodent's models, PRO and CONS, achievement, challenge.

Technical insights are discussed based on practical cases, in-house results and bibliographic analysis. The interactive discussion groups allow attendees to expose and discuss with experts their own scientific issues and give crucial skill to progress in their projects.

Objectives:

  • Learn more about gene editing and how it works
  • Hear about the current advances on many technical aspects
  • Optimize the RNA guide design to the genotyping analysis (bioinformatics work shop)
  • Highlight crucial issues in your own scientific project

Course details and registration

 

If you can´t make it this year: The next training course in gene editing will be offered by Phenomin in autumn 2019 (October 9 - 10).

 

Past courses

14-15 March 2018: In vivo CRISPR/Cas9 genome editing, Strasbourg, France
  • Learn about gene editing and how it works
  • Hear about the current advances on many technical aspects
  • Optimize the RNA guide design to the genotyping analysis (bioinformatics work shop)
  • Highlight crucial issue in your own scientific project

Further information

3-4 April 2017: 2nd programmable nucleases (CRISPR/Cas9) transgenesis course, Prague, Czech Republic

This course, jointly provided by the Czech Centre for Phenogenomics and Phenomin/ICS Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.

Course highlights

  • Design targeting strategy for your project of interest
  • Apply the targeting tools and validate CRISPR activity
  • Deliver the editing tools to cell lines and zygotes
  • Identify CRISPR induced genomic modifications

Place: Czech Centre for Phenogenomics, Vestec/Prague, Czech Republic
Application deadline: 15th February 2017 (results posted 20th February 2017)
Participants: A maximum of 15 participants including 2 scholarships.
Course fee: 980 € for participants from academic institutions and universities.

The cost for participants from industry is available on request. Participation on the course is subject to a selection process. Applications must be accompanied by a short CV, letter of motivation (max 200 words), and a short letter of support. Accommodation and transport costs are not included in the fee and has to be covered by participants on their own. Lunches and refreshments during the course as well as joint dinners are included. Participants wishing to apply for the scholarship should state this clearly in the cover letter. Please note that the scholarship will cover the course fee only.

Applications should be sent to the following email address: ccp-tam@img.cas.cz

Further information on course program and faculty on webpage or download.