Phenotyping training courses
INFRAFRONTIER and its partners offer unique training courses and workshops on high-throughput and on specialised secondary phenotyping technologies. In these courses, the biomedical research community can benefit directly from the outstanding expertise available in the European mouse clinics.
The courses are open to the whole scientific community with a specific interest in mouse phenotyping and may target both scientists and technicians. Animal welfare aspects are an important component of the complete course program.
This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.
- Acquire primary expertise in mouse development
- Be able to design primary phenotyping experiment on mouse embryos
- Learn about all the primary phenotyping analysis on mouse embryos
- Be able to dissect post-implanted embryos
- Be able to determine the window of lethality in utero
- Be able to evaluate embryos viability at neonatal stage
- Increase awareness of relevant techniques to image embryos
6 to 8 graduate students (PhD), post-doctoral scientists, researchers, and engineers
Attendees should have the basic knowledge in mouse physiology and should be able to handle mice.
For details and registration: please click here!
Would you like to learn how to choose, create and analyse your best mouse model?
Do you have a sufficient background in mouse functional genomics to lead as best your research?
PHENOMIN, in collaboration with INFRAFRONTIER, supports researchers who use mouse models by giving us the opportunity to take part to the biennial Edition of the Advanced School in Mouse Phenogenomics. Come to the heart of Europe to be involved - together with other students and researchers from all over the world - in opened discussion, in constructive analysis of your own research and in building conceptual scientific advances.
The school covers the different fields of mouse phenogenomics, ranging through 5 topics from state-of-the-art mouse genetics, mutagenesis, mouse colony management, phenotyping human diseases and data management (statistical approaches). This unique international event will provide the attendees with a wealth of information on good practices in the use of mouse model (experimental design, ethics, regulations, analysis, data and resources ...) to support attendees in their research.
Essentials for accurate interpretation of Precision Medicine models
Although human and mouse have similar anatomical complexity, in general, the level of morphological knowledge is lower in mice than in men.
Nevertheless, an accurate interpretation of Precision Medicine mouse models requires a considerable degree of knowledge in mouse anatomy, histology, and imaging, as well as their comparison with human.
The course consists of two modules:
Module 1 "Anatomy and Imaging" has been held in Barcelona (18-23 June 2018). Applications could be sent to: firstname.lastname@example.org
Module 2 "Histology" will be taught in Prague (03-06 September 2018). Deadline for application was 31 July 2018. Applications should be sent to: email@example.com
Depending on their interest, participants can attend the two modules independently.
Lectures will be followed by practical sessions in which participants will dissect specifically the different organs of the mouse body and will work with bone and dissected specimens, radiographs, and images from TEM, micro-CT and MRI, both from mouse and human. For histological practical sessions participants will perform a complete mouse necropsy. They will take tissue samples, fix and process them, and perform a routine Hematoxilin & Eosin stain on paraffin sections. Virtual slides will be interpreted at the end of each histological lecture.
For the detailed course program - please click here !
TUITION FEE: 550 € for each module
Participants who register for both modules will have 100 € discount.
- Training Course in English
- Training course for engineers and researchers
- This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development.
- The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.
- Learn good practices (3 workshops)
Focus on brown fat metabolism
Partners of INFRAFRONTIER, the European Research Infrastructure for phenotyping and archiving of model mammalian genomes, offer an excellent training opportunity in using innovative technology for rodent body temperature telemetry. The workshop will be hosted by the Czech Centre for Phenogenomics, and will be run by renowned experts of the field. The training workshop will involve presentations on energy and brown fat metabolism from the level of molecular and cellular functions, metabolomics up to whole body energy balance regulation, an overview of the TSE Stellar technology and state-of-the-art implantation surgery including analgesia and animal welfare aspects. The workshop will facilitate extensive discussions of the innovative Stellar technology and its applications, including experimental design and data analyses.
Workshop program attached
Do you have a new PhD student or Post Doc?
MRC Harwell are running a post graduate level course on mouse genetics and phenotyping. This will give them an introduction to the principles, techniques and challenges of mouse genetics, covering:
- Transgenics & CRISPR
- Phenotyping tests
- Bioinformatics tolls
- Breeding strategies
- Development to ageing
- Disease models
Contact us for more information or to register firstname.lastname@example.org
Would you like to learn how to choose, create and analyse your best mouse model? Do you have a sufficient background in mouse functional genomics to lead as best your research?
This international training of excellence provides engineers, PhDs and researchers with an innovative program focused on best practices in the use of the mouse model biomedical and scientific research. Reach us and you will gain crucial skills required to use as best mutant mice and support you in your research!
Registration open! Early bird dead line : March 31st
This unique workshop provides the introduction and practical hands-on of integrated metabolomics workflows, employing the techniques of cellular energy metabolism (Agilent Seahorse XF), detailed metabolomic analysis by LC/MS Q-TOF and the translation of cell culture results into a living mouse model using indirect calorimetry. Results from integrated experimental model, employing all the three techniques will be presented.
- Introduction into experimental workflow design
- Assay of cellular metabolism in real-time
- Mass spectrometry metabolomics
- Indirect calorimetry technique
Practical hands-on in groups:
- Agilent Seahorse XFp
- Agilent LC/MS Q-TOF
- Indirect Calorimetry
Place: Czech Centre for Phenogenomics, Vestec/Prague, Czech Republic
Application deadline: 20 April 2017
Participants: A maximum of 30 participants
Course fee: 50 € (includes: coffeebreaks, lunches, social dinner, reagents & consumables for hands-on session, participant bag)
- Understand the interest of phenotyping to characterize gene function in mouse model for diseases
- Learn about the scientific and ethical considerations in phenotyping
- Hear about the current web resources available
- Learn good practices by experimentation (5 workshops)
[toggle title="10-13 October 2016: INFRAFRONTIER-I3 / Mouse Metabolic Phenotyping Training Course, German Mouse Clinic, Helmholtz Centre Munich, Germany"]
INFRAFRONTIER, the European Research Infrastructure for phenotyping and archiving of model mammalian genomes, offers an excellent training opportunity in mouse metabolic phenotyping. The course will be run by the German Mouse Clinic (GMC) based at the Helmholtz Centre Munich (https://www.mouseclinic.de/index.html), and will cover state of the art phenotyping assays used in the energy metabolism, diabetes, clinical chemistry and pathology screens of the GMC. All assays are routinely applied in systemic phenotyping projects of the GMC, and in part also in the phenotyping pipeline of the International Mouse Phenotyping Consortium (IMPC). The course will be run by highly experienced scientists of the GMC, and involve presentations and extensive discussions of assays, experimental design and data analyses.
Day 1 / October 10th / starting at 12.00
- German Mouse Clinic phenotyping pipelines, INFRAFRONTIER
- Glucose metabolism, glucose tolerance test
- Clamp technologies
Day 2 / October 11th
- Metabolic phenotyping
- Clinical chemistry / liver enzymes
- Pathology and histological analyses
- Tour of German Mouse Clinic
Day 3 / October 12th
- Phenotyping pipeline design
- Data analysis, statistics
- Liver spectroscopy, Maldi mass spectrometry
- Breath gas analysis
- Tour of Helmholtz Genome Analysis Centre (GAC)
Day 4 / October 13th / ending at 17.00
- Pigs as animal models for metabolic research
- Indirect calorimetry
- Human respirometry
- The 4-day course is intending to give participants a comprehensive overview of state of the art technologies and approaches in mouse metabolic phenotyping
- A strong focus will be on experimental design and phenotyping data analyses
- Course level will be at MS, PhD and Postdoc level. No prior experience with metabolic phenotyping required
- The training course is limited to a total of 10 participants
- Cost: 500€ covering course materials, accommodation, coffee breaks, lunch and course dinner (but excluding travel cost)
- Accommodation for course participants has been arranged in the Kurfürst Hotel close to the meeting venue, http://www.kurfuerst-hotel.de/
- Registration: Please submit the registration form with contact details and a short description of your current research interests related to mouse metabolic phenotyping and your training needs
- Registration deadline: September 16th, 2016
- Contact for further enquiries and submission of applications: email@example.com
- Dr Helmut Fuchs (GMC, Helmholtz Zentrum München)
- Dr Valerie Gailus-Durner (GMC, Helmholtz Zentrum München)
- Dr Birgit Rathkolb (GMC, Helmholtz Zentrum München)
- Dr Julia Calzada-Wack (GMC, Helmholtz Zentrum München)
- Dr Patricia da Silva-Buttkus (GMC, Helmholtz Zentrum München)
- Dr Jan Rozman (GMC, Helmholtz Zentrum München)
- Dr Holger Maier (GMC, Helmholtz Zentrum München)
- Martin Kistler (GMC, Helmholtz Zentrum München)
- Mark Haid (GMC, Helmholtz Zentrum München)
- Dr Axel Walch (Helmholtz Zentrum München)
- Dr Michael Hagn (INFRAFRONTIER)
- Dr Simone Renner (Ludwig Maximilian University, Munich)
- Dr Harm Knot (TSE Systems GmbH)
- Dr Luciana Caminha-Afonso
- Dr Kristin Moreth, Dr Michael Hagn (Helmholtz Zentrum München)
- Afonso / MRI and MRS
- Calzada-Wack / Pathology
- Fuchs / Data analysis and statistics
- Fuchs / German Mouse Clinic
- Glaser / DZD
- Hagn / INFRAFRONTIER RI
- Haid / Metabolomics
- Keppner / Human respirometry
- Knot / Energy expenditure and metabolic phenotyping
- Knot / PhenoMaster
- Kistler / VOCs
- Rathkolb / Clinical chemistry for metabolic phenotyping
- Rathkolb and Rozman / Phenotyping glucose metabolism
- Renner / Pigs
- Rozman / Part 1
- Walch / MALDI imaging
UAB offers the following course formats:
- Course on mouse osteology: basis for X-ray and CT bone phenotyping
- Course on mouse blood and lymphatic vessel phenotyping
UAB training course on mouse blood and lymphatic vessel phenotyping
The training course on mouse osteology was held at the UAB on 20 - 22 November 2013 in Barcelona. The UAB course on mouse blood and lymphatic vessel phenotyping is now open for registrations and will be held at the UAB on 17 - 20 November 2014.
Objective of the course
The aim of this Infrafrontier Training Course is capacitating participants to localize and to interpret the most common vascular morphological abnormalities found in mutant mice. This will occur in the context of dedicated learning sessions devoted to the study of blood and lymphatic vessels anatomy, and the methodologies (Dissection, Vascular injection, Corrosion, Transparentation, CT, MRI, Transmission and Scanning Electron Microscopy, Immunohistochemistry, etc.) used to visualize the mouse circulatory system. Lectures will be followed by practical sessions in which participants will demonstrate and analyze mouse vessels with different technologies. In addition to the theoretical and practical sessions, the course will include seminars where specific vascular abnormalities found in mutant mice will be discussed.
Jesús Ruberte, Professor of Veterinary Anatomy. Head of the Mouse Imaging Platform and Morphological Analysis Unit, CBATEG, UAB
Registration dead line
15 October 2014
The organization will cover coffee breaks, lunches (on 17, 18, 19 and 20 November 2014) and the dinner on 19 November 2014 for all participants.
Registration fees: 1200 €
Maximum number of participants: 10
For inscription contact: firstname.lastname@example.org