B6.Cg-Gt(ROSA)26Sortm1Hjf/Ieg

Status

Available to order

EMMA IDEM:02112
International strain nameB6.Cg-Gt(ROSA)26Sortm1Hjf/Ieg
Alternative nameRosa-tdRFP
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolGt(ROSA)26Sortm1Hjf,
Gene/Transgene symbolGt(ROSA)26Sor

Information from provider

ProviderHans Joerg Fehling
Provider affiliationUniversity Clinics Ulm, Institute for Immunology
Genetic informationA targeting vector was designed to contain the "tandem-dimer red fluorescent protein" gene (kind gift of R. Tsien/San Diego) in opposite orientation to Gt(ROSA)26Sor transcription and downstream of a loxP-flanked stop sequence (promoterless neomycin resistance gene and three copies of the bovine growth hormone polyadenylation sequence). This entire construct was inserted into the Gt(ROSA)26Sor locus via gene targeting of C57BL/6-derived Bruce 4 embryonic stem cells.
Phenotypic informationMice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous females exhibit a somewhat reduced fertility when compared with C57BL/6 wildtype animals. The mice contain a tandem-dimer red fluorescent protein (tdRFP) gene inserted in opposite transcriptional orientation into the Gt(ROSA)26Sor locus. Expression of tdRFP is prevented by an upstream loxP-flanked STOP sequence. When bred to mice expressing a cre recombinase gene under the control of a promoter of interest, the tdRFP cassette is irreversibly flipped and the STOP sequence deleted, resulting in tdRFP expression in the cells/tissues of interest. These mutant mice may be useful in monitoring cre recombinase expression in living tissues cytofluorimetrically and histologically. They have been generated in particular for multicolor lineage tracing studies in combination with GFP-based fluorescent proteins.
Breeding historyThe mutation was generated by targeting the appropriate construct into the Gt(ROSA)26Sor locus of C57BL/6-derived Bruce4 embryonic stem cells. Correctly targeted ES cells were microinjected in BALB/c blastocysts. One chimeric animal was bred to a C57BL/6 mouse. Mutant progeny were intercrossed to generate homozygous mutants. The strain is maintained by intercrossing homozygous mutants.
References
  • Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.;Luche Hervé, Weber Odile, Nageswara Rao Tata, Blum Carmen, Fehling Hans Jörg, ;2007;European journal of immunology;37;43-53; 17171761

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Literature references

  • Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.;Luche Hervé, Weber Odile, Nageswara Rao Tata, Blum Carmen, Fehling Hans Jörg, ;2007;European journal of immunology;37;43-53; 17171761

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).