B6;CBA-Tg(Igf2/LacZ,H19)YZ20Aco/AcoH

Status	Available to order
EMMA ID	EM:02378
International strain name	B6;CBA-Tg(Igf2/LacZ,H19)YZ20Aco/AcoH
Alternative name	YZ20
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Igf2/LacZ,H19)YZ20Aco
Gene/Transgene symbol	Tg(Igf2/LacZ,H19)YZ20Aco

Information from provider

Provider	Justin Ainscough
Provider affiliation	Central Biomedical Services, University of Leeds
Genetic information	The YZ20 line (three copy transgene) contains an additional 130 kb genomic region from mouse distal chromosome 7 spanning from Igf2 to ~40 kb downstream of H19. Igf2 has been inactivated by insertional mutagenesis resulting in an Igf2-LacZ gene fusion. A 1kb conserved intergenic region is flanked with LoxP sites. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. The Igf2-LacZ gene does not show appropriate reciprocal imprinting as it is expressed from both parental alleles.
Phenotypic information	The line has not been kept homozygous due to reduced fertility.
References	A skeletal muscle-specific mouse Igf2 repressor lies 40 kb downstream of the gene.;Ainscough J F, John R M, Barton S C, Surani M A, ;2000;Development (Cambridge, England);127;3923-30; 10952890

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

A skeletal muscle-specific mouse Igf2 repressor lies 40 kb downstream of the gene.;Ainscough J F, John R M, Barton S C, Surani M A, ;2000;Development (Cambridge, England);127;3923-30; 10952890

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
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