STOCK Tg(Prnp/PRNP*AHQ)EM16Pcg/H
| Status | Available to order |
| EMMA ID | EM:04339 |
| International strain name | STOCK Tg(Prnp/PRNP*AHQ)EM16Pcg/H |
| Alternative name | Tg(OvPrP)EM16 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Prnp/PRNP*AHQ)EM16Pcg |
| Gene/Transgene symbol | Tg(Prnp/PRNP*AHQ)EM16Pcg |
Information from provider
| Provider | Peter Griffiths |
| Provider affiliation | Department of Pathology and Host Susceptibility, Animal Health and Veterinary Laboratories Agency (AHVLA) |
| Genetic information | A ~12 kb transgene, comprised of chimaeric DNA sequence encoding a sheep PrP (AHQ allele) coding region and mouse PrP gene promoter and 3' UTR, was used to microinject pronuclear stage FVB/N fertilised oocytes. |
| Phenotypic information | Overexpression of transgenic ovine PrPC in brain and other tissues in order to confer increased susceptibility to TSE diseases compared to wild-type mice. |
| Breeding history | Inbred to 7 generations. Brother and sister matings. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous , homozygous |
| Breeding at archiving centre | None |
Literature references
- Overexpression of chimaeric murine/ovine PrP (A136H154Q171) in transgenic mice facilitates transmission and differentiation of ruminant prions.;Griffiths Peter C, Plater Jane M, Chave Alun, Jayasena Dhanushka, Tout Anna C, Rice Paul B, Vickery Christopher M, Spiropoulos John, Stack Michael J, Windl Otto, ;2013;The Journal of general virology;94;2577-2586; 23761404