B6.Cg-Abca3tm1Holz/Ieg

Status

Available to order

EMMA IDEM:04425
International strain nameB6.Cg-Abca3tm1Holz/Ieg
Alternative nameAbca3tm1Holz
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolAbca3tm1Holz,
Gene/Transgene symbolAbca3

Information from provider

ProviderAndreas Holzinger
Provider affiliationKinderklinik, Molekulare Neonatologie, Klinikum der LMU Muenchen
Additional ownerStrain owned (50% each) by Andreas Holzinger, LMU München and Martin Hrabé de Angelis, Helmholtz-zentrum München
Genetic informationExons 5 (partially) to 7 (partially) of the Abca3 gene are deleted and replaced by (5' to 3') an IRES, humanized Renilla green fluorescent protein (hrGFP) cDNA and a neomycin expression cassette. The neo cassette is flanked by loxP sites but still retained in this mouse line.
Phenotypic informationHomozygosity is associated with neonatal respiratory failure due to a disturbance in pulmonary surfactant homeostasis. Heterozygous mice are apparently healthy but do display abnormalities in biochemical analyses and challenge tests.
Breeding historyBackcrossed to C57BL/6 over at least 10 generations.
References
  • Targeted inactivation of the murine Abca3 gene leads to respiratory failure in newborns with defective lamellar bodies.;Hammel Markus, Michel Geert, Hoefer Christina, Klaften Matthias, Müller-Höcker Josef, de Angelis Martin Hrabé, Holzinger Andreas, ;2007;Biochemical and biophysical research communications;359;947-51; 17577581
Homozygous fertileyes
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6N, wild-type C57BL/6N
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • atelectasis / MGI
  • cyanosis / MGI
  • respiratory failure / MGI
  • respiratory distress / MGI
  • abnormal type II pneumocyte morphology / MGI
  • abnormal surfactant physiology / MGI
  • lethargy / MGI
  • abnormal alveolar lamellar body morphology / MGI
  • neonatal lethality, complete penetrance / MGI

Literature references

  • Targeted inactivation of the murine Abca3 gene leads to respiratory failure in newborns with defective lamellar bodies.;Hammel Markus, Michel Geert, Hoefer Christina, Klaften Matthias, Müller-Höcker Josef, de Angelis Martin Hrabé, Holzinger Andreas, ;2007;Biochemical and biophysical research communications;359;947-51; 17577581

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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