MSOSLOW/Orl
| Status | Available to order |
| EMMA ID | EM:04845 |
| International strain name | MSOSLOW/Orl |
| Alternative name | MSO-Slow |
| Strain type | Spontaneous |
| Allele/Transgene symbol | MSO-Slow |
| Gene/Transgene symbol | MSO-Slow |
Information from provider
| Provider | Jean-François Cloix |
| Provider affiliation | Neurobiology Laboratory, University of Orleans |
| Genetic information | Genetic selection of MSO-Slow mice based upon their very long latency toward seizures by injection of a single dose of 75 mg/kg methionine sulfoximine. The selection was performed on genetic random crosses of eight inbred mouse strains: ABP/Le, A/J, BALB/c, C3H/HeJ, C57BL/6J, CBA/H, DBA/2J, and SWXL-4. |
| Phenotypic information | MSO-Slow line is characterized by 1) a very long latency and a very low susceptibility toward seizures upon injection of methionine sulfoximine; 2) low susceptibility to kainic acid and MK-801; 3) an increase in brain glycogen content induced by methionine sulfoximine; 4) involvement of 5-HT in seizures and brain glycogen content. This line has its counterpart as the MSO-Fast mice. |
| Breeding history | Random crosses of eight inbred mouse strains: ABP/Le, A/J, BALB/c, C3H/HeJ, C57BL/6J, CBA/H, DBA/2J, and SWXL-4 to generate G1. G1 was challenged by a single ip injection of 75 mg/kg methionine sulfoximine and selected upon latency to methionine sulfoximine induced seizures. Mice having the longest latency were inter-crossed without brother-sister crosses. This challenge was repeated for 10 generations, reaching the point where the phenotype was considered as fixed. Then crossing sisters with brothers for 8 generations to perform inbreeding. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous 0, homozygous 0 |
| Breeding at archiving centre | Strain with no genotyping |
Literature references
- Selection of two lines of mice based on latency to onset of methionine sulfoximine seizures.;Cloix Jean-François, Tahi Zahir, Martin Benoît, Hévor Tobias, ;2010;Epilepsia;51;118-28; 20015245