STOCK Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon/Flmg
| Status | Available to order |
| EMMA ID | EM:04971 |
| International strain name | STOCK Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon/Flmg |
| Alternative name | TgLrT-HAHuRL.632 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(LYZ-rtTA2S*M2, tetO-ELAVL1)632Dkon |
| Gene/Transgene symbol | Tg(LYZ-rtTA2S*M2,tetO-ELAVL1)632Dkon |
Information from provider
| Provider | Dimitris KONTOYIANNIS |
| Provider affiliation | Immunology, B.S.R.C. Alexander Fleming |
| Genetic information | The complete cDNA sequence for human ELAVL1 (HuR) was obtained from the IMAGE clone number 2901220 (GenBank accession number BC003376) and was subcloned in-frame to an HA-epitope tag for the expression of an N-terminally tagged form. Subsequently, the HA-HuR cDNA was used for the generation of a transgenic construct that contained, in a 5' to 3' orientation, seven copies of the 42 bp tet operator sequence upstream of the minimal CMV promoter (Clontech); a globin intronic sequence for optimal transgene expression; the HA-HuR cDNA; and a bovine growth hormone polyadenylation signal. For the production of transgenic mice, fertilized (CBA x C57BL/6)F2 hybrid zygotes were coinjected with the transgenic devices using standard procedures. |
| Phenotypic information | Macrophage-restricted, Dox-inducible expression, not on polymorphonuclear leukocytes (PMNs) - high expression. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous CBA x C57BL/6, wild-type (CBA x C57BL/6)F1 |
| Stage of embryos | 2-cell |
Literature references
- HuR as a negative posttranscriptional modulator in inflammation.;Katsanou Vicky, Papadaki Olympia, Milatos Stavros, Blackshear Perry J, Anderson Paul, Kollias George, Kontoyiannis Dimitris L, ;2005;Molecular cell;19;777-89; 16168373