STOCK Cdkn2atm1Cjs Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt

Status

Available to order

EMMA IDEM:04996
International strain nameSTOCK Cdkn2atm1Cjs Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt
Alternative nameCtv-a Arf-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn2atm1Cjs,
Gene/Transgene symbolCdkn2a

Information from provider

ProviderLene Uhrbom
Provider affiliationDepartment of Neuroscience, Uppsala University
Additional ownerLene Uhrbom, Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Genetic informationTransgenic construct: pCNPase-TV-A-IRES-lacZ made in FVB/N. Crossed with strain containing targeted deletion p19Arf (exon 1b of the cyclin-dependent kinase inhibitor 2A gene).
Phenotypic informationAllows for infection of 2',3'-cyclic nucleotide 3' phosphodiesterase (CNPase)-expressing cells by RCAS virus. The deletion of exon 1b in the cyclin-dependent kinase inhibitor 2a gene results in complete loss of p19Arf but not p16Ink4a.
Breeding historyThe TV-A founder mouse was crossed with FVB/N and subsequently intercrossed to establish homozygotes. The p19Arf null mice on C57BL/6 background were previously crossed with another transgenic mouse on FVB/N background. These were crossed with FVB/N for two generations to isolate the p19Arf genotype solely and then intercrossed once to generate p19Arf-/- mice. Following, TV-A transgenic mice were crossed with p19Arf null mice and subsequently F1 progeny were crossed to generate mice homozygous for TV-A and p19Arf deletion.
References
  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
  • Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF.;Kamijo T, Zindy F, Roussel M F, Quelle D E, Downing J R, Ashmun R A, Grosveld G, Sherr C J, ;1997;Cell;91;649-59; 9393858
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivinghomozygous , wild-type FVB/N
Breeding at archiving centreNo breeding.
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased papilloma incidence / MGI
  • increased carcinoma incidence / MGI
  • increased incidence of tumors by chemical induction / MGI
  • blindness / MGI
  • abnormal sperm number / MGI
  • testicular atrophy / MGI
  • decreased testis weight / MGI
  • abnormal retinal photoreceptor morphology / MGI
  • microphthalmia / MGI
  • abnormal lens morphology / MGI
  • abnormal retina morphology / MGI
  • increased tumor incidence / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased fibrohistocytoma incidence / MGI
  • retinal detachment / MGI
  • abnormal lens capsule morphology / MGI
  • abnormal retinal inner nuclear layer morphology / MGI
  • increased incidence of tumors by ionizing radiation induction / MGI
  • abnormal retinal ganglion layer morphology / MGI
  • vision/eye phenotype / MGI
  • abnormal cell physiology / MGI
  • primary vitreous hyperplasia / MGI
  • increased fibrosarcoma incidence / MGI
  • persistent hyperplastic primary vitreous / MGI
  • increased fibroblast proliferation / MGI
  • increased lymphoma incidence / MGI
  • increased metastatic potential / MGI
  • increased tumor growth/size / MGI
  • increased squamous cell carcinoma incidence / MGI

Literature references

  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
  • Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF.;Kamijo T, Zindy F, Roussel M F, Quelle D E, Downing J R, Ashmun R A, Grosveld G, Sherr C J, ;1997;Cell;91;649-59; 9393858

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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