STOCK Cdkn2atm1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt

Status

Available to order

EMMA IDEM:04997
International strain nameSTOCK Cdkn2atm1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt
Alternative nameCtv-a Ink4a-Arf-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn2atm1Rdp,
Gene/Transgene symbolCdkn2a

Information from provider

ProviderLene Uhrbom
Provider affiliationDepartment of Neuroscience, Uppsala University
Additional ownerLene Uhrbom, Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Genetic informationTransgenic construct: pCNPase-TV-A-IRES-lacZ made in FVB/N. Crossed with strain containing targeted deletion p16Ink4a and p19Arf (exons 2 and 3 of the cyclin-dependent kinase inhibitor 2a gene).
Phenotypic informationAllows for infection of 2',3'-cyclic nucleotide 3' phosphodiesterase (CNPase)-expressing cells by RCAS virus. The deletion of exons 2 and 3 in the cyclin-dependent kinase inhibitor 2a gene results in complete loss of both p16Ink4a and p19Arf.
Breeding historyThe TV-A founder mouse was crossed with FVB/N and subsequently intercrossed to establish homozygotes. The p16Ink4a-p19Arf null mice on C57BL/6 background were previously crossed with another transgenic mouse on FVB/N background. These were crossed with FVB/N for two generations to isolate the p16Ink4a-p19Arf genotype solely and then intercrossed once to generate p16Ink4a-p19Arf-/- mice. Following, TV-A transgenic mice were crossed with p16Ink4a-p19Arf null mice and subsequently F1 progeny were crossed to generate mice homozygous for TV-A and p16Ink4a-p19Arf deletion.
References
  • Role of the INK4a locus in tumor suppression and cell mortality.;Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho R A, ;1996;Cell;85;27-37; 8620534
  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous 0, wild-type FVB/N
Breeding at archiving centreno breeding at Karolinska Inst.
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal retinal photoreceptor morphology / MGI
  • microphthalmia / MGI
  • cataract / MGI
  • abnormal posterior eye segment morphology / MGI
  • abnormal lens development / MGI
  • abnormal retinal neuronal layer morphology / MGI
  • persistent hyperplastic primary vitreous / MGI
  • increased brain tumor incidence / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal spleen morphology / MGI
  • increased tumor incidence / MGI
  • increased B cell derived lymphoma incidence / MGI
  • increased sarcoma incidence / MGI
  • abnormal spleen red pulp morphology / MGI
  • abnormal cell cycle / MGI
  • increased hemangiosarcoma incidence / MGI
  • increased spleen white pulp amount / MGI
  • increased cutaneous melanoma incidence / MGI
  • increased fibrosarcoma incidence / MGI
  • increased fibroblast proliferation / MGI
  • abnormal keratinocyte physiology / MGI
  • increased squamous cell carcinoma incidence / MGI
  • abnormal skin physiology / MGI
  • abnormal cell physiology / MGI
  • delayed cellular replicative senescence / MGI
  • premature death / MGI
  • increased lymphoma incidence / MGI

Literature references

  • Role of the INK4a locus in tumor suppression and cell mortality.;Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho R A, ;1996;Cell;85;27-37; 8620534
  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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