| Status | Available to order |
| EMMA ID | EM:05328 |
| International strain name | STOCK Mir144/Mir451atm1.2Doca/Cnrm |
| Alternative name | Mir144/Mir451 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Mir144/Mir451atm1.2Doca, |
| Gene/Transgene symbol | Mir144 |
| Provider | Dónal O |
| Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
| Genetic information | An FRT flanked neo cassette with a 5' loxP site was inserted upstream of the transcription initiation site (TSS) for both Mir144 and Mir451. An additional loxP site was inserted between the two microRNAs. Flp mediated recombination removed the neo cassette leaving the TSS floxed. Cre-mediated recombination simultaneous deleted the hairpin that encodes miR-144 as well as the TSS for the miR-144/451 cluster, leading to the loss of both miR-144 and miR-451 expression. |
| Phenotypic information | Mice deficient for the miR-144/451 cluster display a cell autonomous impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. |
| Breeding history | Upon germ line transmission, the mice carrying the targeted allele were crossed to CRE-expressing transgenic mice (Del-Cre) to simultaneous remove the frt flanked neor cassette, the transcription start site, and miR-144. The resulting mutant mice were backcrossed to C57BL/6N to remove the CRE transgene. The offspring carrying the mutation were backcrossed 3 generations to C57BL/6N. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
