MGI phenotypes (allele matching)
- decreased testis weight / MGI
STOCK Piwil4tm2.1Doca/Cnrm
| Status | Available to order |
| EMMA ID | EM:05454 |
| International strain name | STOCK Piwil4tm2.1Doca/Cnrm |
| Alternative name | Miwi2 null |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Piwil4tm2.1Doca, |
| Gene/Transgene symbol | Piwil4 |
Information from provider
| Provider | Donal O'Carroll |
| Provider affiliation | EMBL Mouse Biology Unit |
| Genetic information | To generate Miwi2 null allele we recombineered a targeting construct in which exon 17 was flanked with loxP sites. Cre-mediated deletion of exon 17 results in out of frame splicing between exon 16 and 18 resulting in stop codons prior to the last exon, targeting the mutant transcript for nonsense mediated decay (NMD). Should a fraction of the mutant transcript escape NMD, a truncated protein would be made that lacks most of the Piwi domain and thus would likely be non-functional. |
| Phenotypic information | Homozygous male mice for Miwi2 null allele are viable but present impairment of spermatogenesis that results in severe infertility. The mutation does not affect female fertility at all. |
| Breeding history | Mice carrying the targeted allele were crossed with Cre expressing transgenic mice (CreeR) to remove the LoxP-flanked NeoR cassette. The resulting offspring was backcrossed to C57BL/6N to remove the Cre. |
| References |
|
| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements.;De Fazio Serena, Bartonicek Nenad, Di Giacomo Monica, Abreu-Goodger Cei, Sankar Aditya, Funaya Charlotta, Antony Claude, Moreira Pedro N, Enright Anton J, O'Carroll Dónal, ;2011;Nature;480;259-63; 22020280