C57BL/6-Tg(Fabp4-CIDEA)67Npe/Kctt
| Status | Available to order |
| EMMA ID | EM:05599 |
| International strain name | C57BL/6-Tg(Fabp4-CIDEA)67Npe/Kctt |
| Alternative name | aP2-hCidea-67 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Fabp4-CIDEA)67Npe, |
| Gene/Transgene symbol | Tg(Fabp4-CIDEA)67Npe |
Information from provider
| Provider | Natasa Petrovic |
| Provider affiliation | Animal Facility, Stockholm University, The Wenner-Gren Institute |
| Genetic information | The aP2-hCidea transgenic mice have been created by the pronuclear injection technique on the C57BL/6 background. A construct (6.6 kb-long) contained the aP2-promoter (5.2 kb) fused to the hCidea cDNA, followed by a 3 prime polyadenylation signal. The location of insertion is unknown. Several lines are available for which the copy numbers are not known. |
| Phenotypic information | In the aP2-hCidea mouse the human Cidea is expressed in both brown and white adipose tissue at levels similar to those of endogenous (mouse) Cidea in brown fat. Protein levels in white fat are very different in the different lines that are available: in white adipose tissue, it is high in line 13, medium-low in line 85 and almost absent in line 67. Therefore, line 67 can be considered as a line that has brown-fat specific overexpression of human Cidea. In humans, Cidea is expressed in white fat where its expression levels are inversely correlated to body weight and also dynamically altered during body weight changes. In mice, Cidea protein is expressed only in brown fat at temperatures below thermoneutrality. We have introduced human Cidea in all fat tissues of transgenic mice; thus, at thermoneutrality, only human Cidea is expressed only in white fat, which fully mimics the human condition. |
| Breeding history | The aP2-hCidea mice are generated on a C57BL/6 background and all the time bred on this background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous 0, wild-type C57BL/6N |
| Breeding at archiving centre | no breeding at Karolinska Inst. |
| Stage of embryos | 2-cell |
Literature references
- Cidea improves the metabolic profile through expansion of adipose tissue.;Abreu-Vieira Gustavo, Fischer Alexander W, Mattsson Charlotte, de Jong Jasper M A, Shabalina Irina G, Rydén Mikael, Laurencikiene Jurga, Arner Peter, Cannon Barbara, Nedergaard Jan, Petrovic Natasa, ;2015;Nature communications;6;7433; 26118629