STOCK Otx2tm1Pas/Cnrm

Status

Available to order

EMMA IDEM:05623
International strain nameSTOCK Otx2tm1Pas/Cnrm
Alternative nameOtx2-Lacz
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolOtx2tm1Pas,
Gene/Transgene symbolOtx2

Information from provider

ProviderAntonio Simeone
Provider affiliationCeinge Biotecnologie Avanzate
Additional ownerPhilippe Brulet, Unité d'Embryologie Moléculaire, Centre National de la Recherche Scientifique (CNRS), Institut Pasteur, Paris, France
Genetic informationPart of the Otx2 gene, containing homeodomain, is replaced with lacZ coding sequence by homologous recombination.
Phenotypic informationBy 9.5 dpc, homozygous mutant embryos are characterized by the absence of forebrain and midbrain regions. From the early to midstreak stages, endomesodermal cells expressing lacZ fail to be properly localized anteriorly.
Breeding historyTargeted mutation was introduced in HM-1 ES cells. Mice were always maintained in a mixed C57BL/6 and DBA/2 genetic background.
References
  • Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation.;Acampora D, Mazan S, Lallemand Y, Avantaggiato V, Maury M, Simeone A, Brûlet P, ;1995;Development (Cambridge, England);121;3279-90; 7588062
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • reduced male fertility / MGI
  • decreased litter size / MGI
  • decreased circulating luteinizing hormone level / MGI
  • decreased testis weight / MGI
  • abnormal gonadotroph morphology / MGI
  • decreased gonadotroph cell number / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • abnormal mesoderm development / MGI
  • abnormal somite development / MGI
  • abnormal somite shape / MGI
  • decreased embryo size / MGI
  • abnormal developmental patterning / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal neural tube morphology / MGI
  • small heart / MGI
  • absent pharyngeal arches / MGI
  • embryonic growth retardation / MGI
  • abnormal primitive node morphology / MGI
  • abnormal amnion morphology / MGI
  • abnormal hindbrain development / MGI
  • absent nasal placodes / MGI
  • absent optic vesicle / MGI
  • prenatal lethality, complete penetrance / MGI
  • absent midbrain / MGI
  • absent forebrain / MGI
  • rostral body truncation / MGI
  • abnormal brain development / MGI
  • abnormal forebrain development / MGI
  • abnormal midbrain development / MGI
  • abnormal first pharyngeal arch morphology / MGI
  • acephaly / MGI
  • abnormal maxillary prominence morphology / MGI

Literature references

  • Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation.;Acampora D, Mazan S, Lallemand Y, Avantaggiato V, Maury M, Simeone A, Brûlet P, ;1995;Development (Cambridge, England);121;3279-90; 7588062

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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