MGI phenotypes (allele matching)
STOCK Ightm5.1Cog/Orl
| Status | Available to order |
| EMMA ID | EM:05659 |
| International strain name | STOCK Ightm5.1Cog/Orl |
| Alternative name | IgH 3'RR-deficient mice |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ightm5.1Cog, |
| Gene/Transgene symbol | Igh |
Information from provider
| Provider | Yves Denizot |
| Provider affiliation | UMR CNRS 6101 |
| Genetic information | Knock out of the 3' IgH RR on the E14 129 ES cell line. Backcross with C57BL/6 mice. |
| Phenotypic information | The immunoglobulin heavy chain locus (IgH) undergoes multiple changes along B-cell differentiation. In progenitor B-cells, V(D)J assembly allows expression of mu heavy chains. In mature B-cells, class switch recombination (CSR) may replace the expressed constant (C)mu gene with a downstream CH gene. Finally, plasma cell differentiation strongly boosts IgH transcription. How the multiple IgH transcriptional enhancers tune these changes is unclear. Deletion of the whole IgH 3 prime regulatory region (3'RR) allows normal maturation until the stage of IgM/IgD expressing lymphocytes, but nearly abrogates CSR to all CH genes. Although plasma cell numbers are unaffected, we reveal the role of the 3'RR into the transcriptional burst normally associated to plasma cell differentiation. |
| Breeding history | Inbred for more than 10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
Literature references
- Genomic deletion of the whole IgH 3' regulatory region (hs3a, hs1,2, hs3b, and hs4) dramatically affects class switch recombination and Ig secretion to all isotypes.;Vincent-Fabert Christelle, Fiancette Remi, Pinaud Eric, Truffinet Véronique, Cogné Nadine, Cogné Michel, Denizot Yves, ;2010;Blood;116;1895-8; 20538806