STOCK Gja5tm2.1Kwi/Cnrm

Status

Available to order

EMMA IDEM:06035
International strain nameSTOCK Gja5tm2.1Kwi/Cnrm
Alternative nameCx40A96S
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGja5tm2.1Kwi,
Gene/Transgene symbolGja5

Information from provider

ProviderKlaus Willecke
Provider affiliationLIMES Institute, University of Bonn
Genetic informationThe coding sequence of Gja5 (Cx40) was replaced by the Cx40A96S coding sequence. The expression of the Cx40A96S mutation is coupled to an eGFP reporter sequence via an IRES sequence. The neomycin resistance gene for screening of ES cell clones was deleted via the flp recombinase/frt sites system.
Phenotypic informationHomozygous Cx40A96S mice exhibit renin-dependent hypertension.
Breeding historyThe line was backcrossed at least 3 times to C57BL/6N.
References
  • The connexin 40 A96S mutation causes renin-dependent hypertension.;Lübkemeier Indra, Machura Katharina, Kurtz Lisa, Neubauer Björn, Dobrowolski Radek, Schweda Frank, Wagner Charlotte, Willecke Klaus, Kurtz Armin, ;2011;Journal of the American Society of Nephrology : JASN;22;1031-40; 21597036
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • no phenotypic analysis / MGI
  • increased circulating renin level / MGI
  • decreased mean systemic arterial blood pressure / MGI

Literature references

  • The connexin 40 A96S mutation causes renin-dependent hypertension.;Lübkemeier Indra, Machura Katharina, Kurtz Lisa, Neubauer Björn, Dobrowolski Radek, Schweda Frank, Wagner Charlotte, Willecke Klaus, Kurtz Armin, ;2011;Journal of the American Society of Nephrology : JASN;22;1031-40; 21597036

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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