STOCK Cdc20tm1.1Mama/Cnbc
| Status | Available to order |
| EMMA ID | EM:06117 |
| International strain name | STOCK Cdc20tm1.1Mama/Cnbc |
| Alternative name | Cdc20 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Cdc20tm1.1Mama, |
| Gene/Transgene symbol | Cdc20 |
Information from provider
| Provider | Marcos Malumbres |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas (CNIO) |
| Genetic information | A loxP site was inserted upstream of exon 2, and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 2; flp-mediated recombination removed the neo cassette leaving exon 2 floxed. |
| Phenotypic information | Before cre-mediated recombination there is no phenotype associated to this allele. |
| Breeding history | Chimeras were crossed with C57BL/6J females for germ line transmission testing. To remove the neoR selection marker Aurkb+/loxfrt mice were crossed with Tg(ACTFLPe)9205Dym mice to remove the frt-flanked neomycin resistance cassette used for homologous recombination selection. Currently maintained by crossing homozygous mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous 0 |
Literature references
- Targeting mitotic exit leads to tumor regression in vivo: Modulation by Cdk1, Mastl, and the PP2A/B55α,δ phosphatase.;Manchado Eusebio, Guillamot María, de Cárcer Guillermo, Eguren Manuel, Trickey Michelle, García-Higuera Irene, Moreno Sergio, Yamano Hiroyuki, Cañamero Marta, Malumbres Marcos, ;2010;Cancer cell;18;641-54; 21156286