STOCK Pmeltm1.1Sson/Kctt

Status

Available to order

EMMA IDEM:06222
International strain nameSTOCK Pmeltm1.1Sson/Kctt
Alternative namePmel KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPmeltm1.1Sson,
Gene/Transgene symbolPmel

Information from provider

ProviderSusanne Kerje
Provider affiliationIMBIM, Uppsala University
Genetic informationExons 2 and 3 of the Pmel 17 gene have been knocked out resulting in a non functional protein.
Phenotypic informationMice lacking PMEL have almost normal fur pigmentation. Loss of PMEL has a dramatic effect on the morphology of the melanosomes in skin, hair, and eye, such that the normally rod-shaped melanosomes in wild-type animals are spherical in the knockout mice. The knockout animals also have a substantial reduction in the content of black pigment in hair.
Breeding historyThe Pmel KO is maintained in a C57BL/6 background.
References
  • Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.;Hellström Anders R, Watt Brenda, Fard Shahrzad Shirazi, Tenza Danièle, Mannström Paula, Narfström Kristina, Ekesten Björn, Ito Shosuke, Wakamatsu Kazumasa, Larsson Jimmy, Ulfendahl Mats, Kullander Klas, Raposo Graça, Kerje Susanne, Hallböök Finn, Marks Michael S, Andersson Leif, ;2011;PLoS genetics;7;e1002285; 21949658
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden

Disease and phenotype information

MGI phenotypes (allele matching)
  • diluted coat color / MGI
  • abnormal melanosome morphology / MGI
  • abnormal tail hair pigmentation / MGI
  • abnormal retinal melanin granule morphology / MGI
  • abnormal choroid melanin granule morphology / MGI

Literature references

  • Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation.;Hellström Anders R, Watt Brenda, Fard Shahrzad Shirazi, Tenza Danièle, Mannström Paula, Narfström Kristina, Ekesten Björn, Ito Shosuke, Wakamatsu Kazumasa, Larsson Jimmy, Ulfendahl Mats, Kullander Klas, Raposo Graça, Kerje Susanne, Hallböök Finn, Marks Michael S, Andersson Leif, ;2011;PLoS genetics;7;e1002285; 21949658

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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