STOCK Tg(Alb-luc/EGFP)5190Ciem/Cnbc
Status | Available to order |
EMMA ID | EM:06345 |
International strain name | STOCK Tg(Alb-luc/EGFP)5190Ciem/Cnbc |
Alternative name | C,B6CBA-Tg(Alb-Luc-eGFP)5190Ciem [Alb1-LUC-eGFP.5190] |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(Alb-luc/EGFP)5190Ciem |
Gene/Transgene symbol | Tg(Alb-luc/EGFP)5190Ciem |
Information from provider
Provider | Jose Carlos Segovia |
Provider affiliation | Differentiation and Cytometry Unit. Hematopoietic and Gene Therapy Division, Centro de Investigaciones Energéticas, Medio Ambientales y Tecnológicas (CIEMAT) |
Genetic information | Luciferase-eGFP fusion gene inserted at ATG start codon of Alb1 mouse locus harbored in BAC clone RP23-27P6 (chromosome 5) by recombineering techniques (160 kpb of this construction injected). The utility of the Alb1-LUC:eGFP transgenic mouse includes (1) Monitoring of hepatic damage/recovery by non-invasive techniques. This would be useful in studies of drug hepatotoxicity/screening; and (2) Tracking of bone marrow donor cells that could contribute to hepatic regeneration in a context of liver injury. Copy number of transgene (QPCR): Alb1-LUC-eGFP.5190: 10 |
Phenotypic information | Alb1-LUC-eGFP.5190: - specific high luciferase activity in liver (less than 1% luciferase activity in other tissues) - specific EGFP staining in cryosections of liver - high in vivo liver specific luminescence detected after treatment with luciferine - high liver-specific epi-fluorescence detected |
Breeding history | Founders are in background B6CBAF2. Current animals (F2) are in the second generation of backcrossing to BALB/c background. |
References | None available |
Homozygous fertile | not known |
Homozygous viable | not known |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | heterozygous 0 |
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