STOCK Tg(Alb-luc/EGFP)5190Ciem/Cnbc

Status

Available to order

EMMA IDEM:06345
International strain nameSTOCK Tg(Alb-luc/EGFP)5190Ciem/Cnbc
Alternative nameC,B6CBA-Tg(Alb-Luc-eGFP)5190Ciem [Alb1-LUC-eGFP.5190]
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Alb-luc/EGFP)5190Ciem
Gene/Transgene symbolTg(Alb-luc/EGFP)5190Ciem

Information from provider

ProviderJose Carlos Segovia
Provider affiliationDifferentiation and Cytometry Unit. Hematopoietic and Gene Therapy Division, Centro de Investigaciones Energéticas, Medio Ambientales y Tecnológicas (CIEMAT)
Genetic informationLuciferase-eGFP fusion gene inserted at ATG start codon of Alb1 mouse locus harbored in BAC clone RP23-27P6 (chromosome 5) by recombineering techniques (160 kpb of this construction injected). The utility of the Alb1-LUC:eGFP transgenic mouse includes (1) Monitoring of hepatic damage/recovery by non-invasive techniques. This would be useful in studies of drug hepatotoxicity/screening; and (2) Tracking of bone marrow donor cells that could contribute to hepatic regeneration in a context of liver injury. Copy number of transgene (QPCR): Alb1-LUC-eGFP.5190: 10
Phenotypic informationAlb1-LUC-eGFP.5190: - specific high luciferase activity in liver (less than 1% luciferase activity in other tissues) - specific EGFP staining in cryosections of liver - high in vivo liver specific luminescence detected after treatment with luciferine - high liver-specific epi-fluorescence detected
Breeding historyFounders are in background B6CBAF2. Current animals (F2) are in the second generation of backcrossing to BALB/c background.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous 0

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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