STOCK Fras1tm1.1Pjsc/H

Status

Available to order

EMMA IDEM:06767
International strain nameSTOCK Fras1tm1.1Pjsc/H
Alternative nameFras1 Conditional
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolFras1tm1.1Pjsc,
Gene/Transgene symbolFras1

Information from provider

ProviderPeter Scambler
Provider affiliationMolecular Medicine Unit, Institute Of child Health
Genetic informationConditional allele of Fras1 with exon 2 flanked by LoxP sites.
Phenotypic informationNo phenotype as homozygous floxed allele; recapitulates constitutive null with ubiquitous Cre driver. The conditional knock-out mice, when crossed with an ubiquitous cre-driving line, recapitulate the phenotype of blebbed mutant (nonsense mutation in Fras1 gene) and constitute a good model of human Fraser syndrome (skin lesions, kidney and upper airway malformations associated with high neomortality). The blebbed mutant is also archived - see EM:02533.
Breeding historyThe targeting vector was electroporated into C57BL/6 mouse ES cells and a clone was used to inject C57BL/6 blastocysts to generate chimeras. Backcrossing to C57BL/6Crl mice produced heterozygous F1 offspring with germline transmission of Fras1flneo. The retained Neo cassette flanked by Frt sites was excised by crossing heterozygous Fras1flneo mice with B6;SJL-Tg(ACTFLPe)9205Dym/J mice. The FLPe transgene was then removed by backcrossing to C57BL/6Crl and mice intercrossed to produce homozygotes. These mice have been backcrossed to C57BL/6Crl three times.
References
  • Generation of mice with a conditional null Fraser syndrome 1 (Fras1) allele.;Pitera Jolanta E, Turmaine Mark, Woolf Adrian S, Scambler Peter J, ;2012;Genesis (New York, N.Y. : 2000);50;892-8; 22730198
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous 0

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • no phenotypic analysis / MGI

Literature references

  • Generation of mice with a conditional null Fraser syndrome 1 (Fras1) allele.;Pitera Jolanta E, Turmaine Mark, Woolf Adrian S, Scambler Peter J, ;2012;Genesis (New York, N.Y. : 2000);50;892-8; 22730198

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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