STOCK Cers4tm1.1Kwi/Cnrm

Status

Available to order

EMMA IDEM:07171
International strain nameSTOCK Cers4tm1.1Kwi/Cnrm
Alternative nameCerS4KILacZ
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCers4tm1.1Kwi,
Gene/Transgene symbolCers4

Information from provider

ProviderKlaus Willecke
Provider affiliationMolecular genetics, LIMES Institute, University of Bonn
Genetic informationThe exons 2-11 of the Cers4 gene were replaced with a LacZ reporter gene followed by a FRT-flanked neo cassette. Flp-mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of protein expression in several tissues. LacZ expression is detected by staining with X-Gal.
Phenotypic informationAltered sphingolipid metabolism, alopecia.
Breeding historyBackcrossed to 93.8% C57BL/6. There occur no problems with homozygous breedings.
References
  • Ceramide synthase 4 deficiency in mice causes lipid alterations in sebum and results in alopecia.;Ebel Philipp, Imgrund Silke, Vom Dorp Katharina, Hofmann Kristina, Maier Helena, Drake Helena, Degen Joachim, Dörmann Peter, Eckhardt Matthias, Franz Thomas, Willecke Klaus, ;2014;The Biochemical journal;461;147-58; 24738593
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal hair follicle morphology / MGI
  • alopecia / MGI
  • enlarged sebaceous gland / MGI
  • thick epidermis / MGI
  • abnormal hair texture / MGI
  • abnormal sebaceous gland physiology / MGI
  • abnormal sebaceous lipid secretion / MGI
  • hair follicle degeneration / MGI
  • progressive hair loss / MGI
  • abnormal sphingomyelin level / MGI
  • abnormal ceramide level / MGI

Literature references

  • Ceramide synthase 4 deficiency in mice causes lipid alterations in sebum and results in alopecia.;Ebel Philipp, Imgrund Silke, Vom Dorp Katharina, Hofmann Kristina, Maier Helena, Drake Helena, Degen Joachim, Dörmann Peter, Eckhardt Matthias, Franz Thomas, Willecke Klaus, ;2014;The Biochemical journal;461;147-58; 24738593

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).