STOCK Cyldtm1.1Gmos/Flmg

Status

Available to order

EMMA IDEM:07405
International strain nameSTOCK Cyldtm1.1Gmos/Flmg
Alternative nameCyldtm1.1Gmos (Cylddelta9)
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCyldtm1.1Gmos,
Gene/Transgene symbolCyld

Information from provider

ProviderGeorge Mosialos
Provider affiliationBiology department, Aristotle University of Thessaloniki
Genetic informationA floxed neo cassette was inserted upstream of exon 9 and an additional loxP site was inserted downstream of exon 9. Cre mediated recombination removed the neo cassette and exon 9. This exon codes for an essential catalytic motif of the deubiquitination domain. The absence of protein expression was confirmed by Western blot analysis on fibroblast extracts.
Phenotypic informationThis is a mouse model of CYLD inactivation in all tissues. Systems affected: cellular (abnormal cell migration) growth/size (decreased body size), homeostasis/metabolism (cyanosis) limbs/digits/tail (kinked tail, short tail) mortality/aging (neonatal lethality) respiratory system (abnormal lung development, abnormal lung interstitium morphology, small lung, abnormal respiratory function, atelectasis).
References
  • Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation.;Trompouki Eirini, Tsagaratou Ageliki, Kosmidis Stylianos K, Dollé Pascal, Qian Jun, Kontoyiannis Dimitris L, Cardoso Wellington V, Mosialos George, ;2009;Neoplasia (New York, N.Y.);11;469-76; 19412431
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archivingheterozygous C57BL/6, wild-type C57BL/6
Stage of embryosMorula

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • kinked tail / MGI
  • short tail / MGI
  • abnormal lung development / MGI
  • atelectasis / MGI
  • cyanosis / MGI
  • abnormal lung interstitium morphology / MGI
  • abnormal respiratory function / MGI
  • small lung / MGI
  • decreased birth body size / MGI
  • neonatal lethality, complete penetrance / MGI
  • thick lung-associated mesenchyme / MGI
  • impaired fibroblast cell migration / MGI

Literature references

  • Truncation of the catalytic domain of the cylindromatosis tumor suppressor impairs lung maturation.;Trompouki Eirini, Tsagaratou Ageliki, Kosmidis Stylianos K, Dollé Pascal, Qian Jun, Kontoyiannis Dimitris L, Cardoso Wellington V, Mosialos George, ;2009;Neoplasia (New York, N.Y.);11;469-76; 19412431

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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