STOCK Rag2tm1Fwa Thy1a Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl

Status

Available to order

EMMA IDEM:07459
International strain nameSTOCK Rag2tm1Fwa Thy1a Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl
Alternative nameRag2-/- Tg HY +/+ Thy1.1
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRag2tm1Fwa,
Gene/Transgene symbolRag2

Information from provider

ProviderBenedita Rocha
Provider affiliationINSERM U 1020, INSERM
Additional ownerFrederick W. Alt, Harald von Boehmer
Genetic informationMutant mice bearing a transgenic alpha-beta TCR specific for the HY male antigen restricted to major histocompatibility complex (MHC) class I H2Db and deficient in the Rag2 recombinase gene (TgRAG2) were used to obtain monoclonal populations of CD8+T cells.
Phenotypic informationMice will have a single population of lymphocytes, those expressing the TCR transgene.
Breeding historyCurrently bred as homozygotes for the Rag2 knock-out mutation and the TCR transgene, Thya (Thy1.1) allotype.
References
  • Differential requirements for survival and proliferation of CD8 naïve or memory T cells.;Tanchot C, Lemonnier F A, Pérarnau B, Freitas A A, Rocha B, ;1997;Science (New York, N.Y.);276;2057-62; 9197272
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedyes

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivinghomozygous C57BL/6J, homozygous C57BL/6J
Breeding at archiving centreHomozygous for the three mutations
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI

Literature references

  • Differential requirements for survival and proliferation of CD8 naïve or memory T cells.;Tanchot C, Lemonnier F A, Pérarnau B, Freitas A A, Rocha B, ;1997;Science (New York, N.Y.);276;2057-62; 9197272

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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