CnbcLmon:NMRI-Tg(tetO-Tyr)1335Lmon Tg(Tyr-rtTA)4111Lmon Gnat2+

Status

Available to order

EMMA IDEM:08317
International strain nameCnbcLmon:NMRI-Tg(tetO-Tyr)1335Lmon Tg(Tyr-rtTA)4111Lmon Gnat2+
Alternative nameTRETyBS/HSTyrTET/Gnat2+
Strain typeOther
Allele/Transgene symbolGnat2+,
Gene/Transgene symbolGnat2

Information from provider

ProviderLluis Montoliu
Provider affiliationMolecular and Cellular Biology, CNB-CSIC
Genetic informationThis strain derives from EMMA mouse strain EM:02611, in which the wild-type allele of the Gnat2 locus (Gnat2+) has been selected, hence producing a new strain. Therefore, this is also a double transgenic mouse line carrying two constructs for doxycycline-mediated tyrosinase gene regulation (TET-ON model), both in homozygous state. This is an animal model for rare disease oculocutaneous albinism type 1 (OCA1). It uses the first Tet system developed by H. Bujard and colleagues, therefore the used version of the transactivator was "Tet On/Off" (Tet On scheme, using rtTA) and the version of the Tet promoter was "PTRE" (see PubMed ID 15250938).
Phenotypic informationHomozygous:
In the off-status (without Dox), externally undistinguishable from a common albino mouse phenotype. Upon Dox treatment, from first gestational week onwards pups will show a darker ruby eye colour. Coat colour is not modified by Dox treatment. Mice do not have the retinal degeneration associated to the Gnat2 mutation because this mouse strain has been selected for the Gnat2<+> allele.

Heterozygous:
Similar to homozygous state.
Breeding historyTransgenic mice were initially generated in FVB/HanHsd albino inbred mice but expanded in HsdWin:NMRI albino outbred mice to avoid the retinal degeneration mutation (Pdebrd1) carried by the FVB genotype. Mice were bred to double transgenic homozygosity and have been maintained since then as double transgenic homozygous in HsdWin:NMRI albino outbred mice (more than 20 generations). Recently, a mutation for the Gnat2 locus was discovered in HsdWin:NMRI background (Gnat2cpfl3) and this new line has been selected for the homozygous wild-type allele (Gnat2+).
References
  • A transgenic mouse model with inducible Tyrosinase gene expression using the tetracycline (Tet-on) system allows regulated rescue of abnormal chiasmatic projections found in albinism.;Giménez Estela, Lavado Alfonso, Giraldo Patricia, Cozar Patricia, Jeffery Glen, Montoliu Lluís, ;2004;Pigment cell research;17;363-70; 15250938
  • New animal models to study the role of tyrosinase in normal retinal development.;Lavado Alfonso, Montoliu Lluis, ;2006;Frontiers in bioscience : a journal and virtual library;11;743-52; 16146766
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous NMRI, homozygous NMRI
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • absent coat pigmentation / MGI
  • diluted coat color / MGI
  • abnormal coat/hair pigmentation / MGI
  • absent eye pigmentation / MGI
  • abnormal coat appearance / MGI
  • decreased eye pigmentation / MGI
  • mottled coat / MGI
  • abnormal eye pigmentation / MGI
  • belly spot / MGI
  • hypopigmentation / MGI
  • variegated eye pigmentation pattern / MGI
  • decreased ear pigmentation / MGI

Literature references

  • A transgenic mouse model with inducible Tyrosinase gene expression using the tetracycline (Tet-on) system allows regulated rescue of abnormal chiasmatic projections found in albinism.;Giménez Estela, Lavado Alfonso, Giraldo Patricia, Cozar Patricia, Jeffery Glen, Montoliu Lluís, ;2004;Pigment cell research;17;363-70; 15250938
  • New animal models to study the role of tyrosinase in normal retinal development.;Lavado Alfonso, Montoliu Lluis, ;2006;Frontiers in bioscience : a journal and virtual library;11;743-52; 16146766

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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