B6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH

Status

Available to order

EMMA IDEM:08395
International strain nameB6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH
Alternative nameR26Fucci2aR
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGt(ROSA)26Sortm1(CAG-Venus/GMNN, Cherry/CDT1)Jkn
Gene/Transgene symbolGt(ROSA)26Sor

Information from provider

ProviderRichard Mort
Provider affiliationDepartment of Medical & Developmental Genetics, MRC Human Genetics Unit
Genetic informationA targeted insertion of CAG-floxNeo-Fucci2a-pA into the Gt(ROSA)26Sor locus on mouse chromosome 6.
Phenotypic informationThe Fucci system was developed by fusing red- and green-emitting fluorescent proteins to the E3 ligase substrates, Cdt1 (chromatin licensing and DNA replication factor 1) and geminin. These fluorescent probes make it possible to distinguish between cells in the G1 or S/G2/M phases of the cell cycle, in live cells/tissue (Sakaue-Sawano A. et al., 2008). Fucci2a is a bicistronic version of Fucci2 allowing cre recombinase-mediated tissue-specific expression in mice (Mort R. et al., 2014).
Breeding historyTargeted in E14 129P2/Ola ES cells. Subsequently backcrossed for ca. 3 generations onto C57BL/6.
References
  • Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.;Mort Richard Lester, Ford Matthew Jonathan, Sakaue-Sawano Asako, Lindstrom Nils Olof, Casadio Angela, Douglas Adam Thomas, Keighren Margaret Anne, Hohenstein Peter, Miyawaki Atsushi, Jackson Ian James, ;2014;Cell cycle (Georgetown, Tex.);13;2681-96; 25486356
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

  • Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.;Mort Richard Lester, Ford Matthew Jonathan, Sakaue-Sawano Asako, Lindstrom Nils Olof, Casadio Angela, Douglas Adam Thomas, Keighren Margaret Anne, Hohenstein Peter, Miyawaki Atsushi, Jackson Ian James, ;2014;Cell cycle (Georgetown, Tex.);13;2681-96; 25486356
  • Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury.;Lazzeri Elena, Angelotti Maria Lucia, Peired Anna, Conte Carolina, Marschner Julian A, Maggi Laura, Mazzinghi Benedetta, Lombardi Duccio, Melica Maria Elena, Nardi Sara, Ronconi Elisa, Sisti Alessandro, Antonelli Giulia, Becherucci Francesca, De Chiara Letizia, Guevara Ricardo Romero, Burger Alexa, Schaefer Beat, Annunziato Francesco, Anders Hans-Joachim, Lasagni Laura, Romagnani Paola, ;2018;Nature communications;9;1344; 29632300
  • Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease.;De Chiara Letizia, Conte Carolina, Semeraro Roberto, Diaz-Bulnes Paula, Angelotti Maria Lucia, Mazzinghi Benedetta, Molli Alice, Antonelli Giulia, Landini Samuela, Melica Maria Elena, Peired Anna Julie, Maggi Laura, Donati Marta, La Regina Gilda, Allinovi Marco, Ravaglia Fiammetta, Guasti Daniele, Bani Daniele, Cirillo Luigi, Becherucci Francesca, Guzzi Francesco, Magi Alberto, Annunziato Francesco, Lasagni Laura, Anders Hans-Joachim, Lazzeri Elena, Romagnani Paola, ;2022;Nature communications;13;5805; 36195583

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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