B6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH
Status | Available to order |
EMMA ID | EM:08395 |
International strain name | B6;129P2-Gt(ROSA)26Sortm1(CAG-Venus/GMNN,-Cherry/CDT1)Jkn/JknH |
Alternative name | R26Fucci2aR |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Gt(ROSA)26Sortm1(CAG-Venus/GMNN, Cherry/CDT1)Jkn |
Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
Provider | Richard Mort |
Provider affiliation | Department of Medical & Developmental Genetics, MRC Human Genetics Unit |
Genetic information | A targeted insertion of CAG-floxNeo-Fucci2a-pA into the Gt(ROSA)26Sor locus on mouse chromosome 6. |
Phenotypic information | The Fucci system was developed by fusing red- and green-emitting fluorescent proteins to the E3 ligase substrates, Cdt1 (chromatin licensing and DNA replication factor 1) and geminin. These fluorescent probes make it possible to distinguish between cells in the G1 or S/G2/M phases of the cell cycle, in live cells/tissue (Sakaue-Sawano A. et al., 2008). Fucci2a is a bicistronic version of Fucci2 allowing cre recombinase-mediated tissue-specific expression in mice (Mort R. et al., 2014). |
Breeding history | Targeted in E14 129P2/Ola ES cells. Subsequently backcrossed for ca. 3 generations onto C57BL/6. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice.;Mort Richard Lester, Ford Matthew Jonathan, Sakaue-Sawano Asako, Lindstrom Nils Olof, Casadio Angela, Douglas Adam Thomas, Keighren Margaret Anne, Hohenstein Peter, Miyawaki Atsushi, Jackson Ian James, ;2014;Cell cycle (Georgetown, Tex.);13;2681-96; 25486356
- Endocycle-related tubular cell hypertrophy and progenitor proliferation recover renal function after acute kidney injury.;Lazzeri Elena, Angelotti Maria Lucia, Peired Anna, Conte Carolina, Marschner Julian A, Maggi Laura, Mazzinghi Benedetta, Lombardi Duccio, Melica Maria Elena, Nardi Sara, Ronconi Elisa, Sisti Alessandro, Antonelli Giulia, Becherucci Francesca, De Chiara Letizia, Guevara Ricardo Romero, Burger Alexa, Schaefer Beat, Annunziato Francesco, Anders Hans-Joachim, Lasagni Laura, Romagnani Paola, ;2018;Nature communications;9;1344; 29632300
- Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease.;De Chiara Letizia, Conte Carolina, Semeraro Roberto, Diaz-Bulnes Paula, Angelotti Maria Lucia, Mazzinghi Benedetta, Molli Alice, Antonelli Giulia, Landini Samuela, Melica Maria Elena, Peired Anna Julie, Maggi Laura, Donati Marta, La Regina Gilda, Allinovi Marco, Ravaglia Fiammetta, Guasti Daniele, Bani Daniele, Cirillo Luigi, Becherucci Francesca, Guzzi Francesco, Magi Alberto, Annunziato Francesco, Lasagni Laura, Anders Hans-Joachim, Lazzeri Elena, Romagnani Paola, ;2022;Nature communications;13;5805; 36195583
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