STOCK Eif2ak4tm1.2Dron/Cnbc

Status

Available to order

EMMA IDEM:09406
International strain nameSTOCK Eif2ak4tm1.2Dron/Cnbc
Alternative nameEif2ak4_tm1.2Dron_GCN2deficient
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolEif2ak4tm1.2Dron,
Gene/Transgene symbolEif2ak4

Information from provider

ProviderJuanjo Berlanga
Provider affiliationGenome Dynamics and Function, Centro de Biología Molecular Severo Ochoa (CSIC-UAM)
Additional ownerDr. David Ron, University of Cambridge, Cambridge, UK
Genetic informationEif2ak4_GCN2-deficient gene (GCN2 allele constitutively missing exon XII (GCN2). Exon XII encodes a region of GCN2 that binds ATP, and its deletion eliminates kinase activity.
Phenotypic informationHomozygous:
GCN2 homozygous mutant mice exhibit a less aversive response towards imbalanced diet, therefore eIF2 kinase GCN2 has an important role in mediating aversion toward foods with an imbalanced amino acid composition. GCN2-induced eIF2 phosphorylation may promote the aversive response through its effects on translation and transcription in cells of the Anterior Piriform Cortex (APC), in other brain regions, and in other peripheral organs.

Heterozygous:
GCN2 heterozygous mutant mice, exhibit a wild-type aversive response toward foods with an imbalanced amino acid composition.
References
  • The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores.;Maurin Anne-Catherine, Jousse Céline, Averous Julien, Parry Laurent, Bruhat Alain, Cherasse Yoan, Zeng Huiqing, Zhang Yuhong, Harding Heather P, Ron David, Fafournoux Pierre, ;2005;Cell metabolism;1;273-7; 16054071
  • Regulated translation initiation controls stress-induced gene expression in mammalian cells.;Harding H P, Novoa I, Zhang Y, Zeng H, Wek R, Schapira M, Ron D, ;2000;Molecular cell;6;1099-108; 11106749
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous 129/Sv (synonym: 129Sv), homozygous 129/Sv (synonym: 129Sv)
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal contextual conditioning behavior / MGI
  • abnormal long term potentiation / MGI
  • abnormal spatial reference memory / MGI
  • abnormal food preference / MGI
  • abnormal dendritic cell physiology / MGI
  • abnormal cytokine secretion / MGI

Literature references

  • The GCN2 kinase biases feeding behavior to maintain amino acid homeostasis in omnivores.;Maurin Anne-Catherine, Jousse Céline, Averous Julien, Parry Laurent, Bruhat Alain, Cherasse Yoan, Zeng Huiqing, Zhang Yuhong, Harding Heather P, Ron David, Fafournoux Pierre, ;2005;Cell metabolism;1;273-7; 16054071
  • Regulated translation initiation controls stress-induced gene expression in mammalian cells.;Harding H P, Novoa I, Zhang Y, Zeng H, Wek R, Schapira M, Ron D, ;2000;Molecular cell;6;1099-108; 11106749

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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