B6N.129P2(Cg)-Stat1tm2.1Bstr/Biat

Status

Available to order

EMMA IDEM:09915
International strain nameB6N.129P2(Cg)-Stat1tm2.1Bstr/Biat
Alternative nameB6.129P2-Stat1alpha
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolStat1tm2.1Bstr,
Gene/Transgene symbolStat1

Information from provider

ProviderMathias Müller
Provider affiliationDepartment for Biomedical Sciences, University of Veterinary Medicine Vienna (Vetmeduni Vienna)
Additional ownerProf. Thomas Rülicke, University of Veterinary Medicine, Institute of Laboratory Animal Science, Vienna, Austria
Genetic informationThe terminal portion of the cDNA for Stat1beta fused in frame into exon 19 of the genomic Stat1 gene (accession no. AF349678). A human beta-globin splice and a floxed neomycin cassette were inserted downstream of the cDNAs and placed between two regions of homology. Offspring carrying the targeted alleles were crossed to Tg(CMV-cre) mice (Schwenk et al. 1995 Nucleic Acids Res. 23:5080–5081) to remove the neomycin cassette and subsequently intercrossed to obtain Stat1 beta/beta homozygous mice.
Phenotypic informationHomozygous:
Stat1 beta/beta mice show more prolonged IFN-gamma-induced phosphorylation and promoter binding; mediates protective, IFN-gamma-dependent immunity against the bacterium Listeria monocytogenes.

Heterozygous:
n/a
Breeding historyThe mice were backcrossed to the C57BL/6N background by speed congenics (Teppner et al. 2004; Lab Anim. 38:406–412. doi:10.1258/0023677041958882).
References
  • STAT1β is not dominant negative and is capable of contributing to gamma interferon-dependent innate immunity.;Semper Christian, Leitner Nicole R, Lassnig Caroline, Parrini Matthias, Mahlakõiv Tanel, Rammerstorfer Michael, Lorenz Karin, Rigler Doris, Müller Simone, Kolbe Thomas, Vogl Claus, Rülicke Thomas, Staeheli Peter, Decker Thomas, Müller Mathias, Strobl Birgit, ;2014;Molecular and cellular biology;34;2235-48; 24710278
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivinghomozygous C57BL/6.Cg

Literature references

  • STAT1β is not dominant negative and is capable of contributing to gamma interferon-dependent innate immunity.;Semper Christian, Leitner Nicole R, Lassnig Caroline, Parrini Matthias, Mahlakõiv Tanel, Rammerstorfer Michael, Lorenz Karin, Rigler Doris, Müller Simone, Kolbe Thomas, Vogl Claus, Rülicke Thomas, Staeheli Peter, Decker Thomas, Müller Mathias, Strobl Birgit, ;2014;Molecular and cellular biology;34;2235-48; 24710278

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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