MF1;129X1-Ralgdstm1Cjm/H
| Status | Available to order |
| EMMA ID | EM:12460 |
| International strain name | MF1;129X1-Ralgdstm1Cjm/H |
| Alternative name | RalGDS KO Mouse |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ralgdstm1Cjm, |
| Gene/Transgene symbol | Ralgds |
Information from provider
| Provider | Chris Marshall |
| Provider affiliation | The Institute of Cancer Research |
| Genetic information | Knockout of RalGDS. Mouse RalGDS genomic clones were obtained by screening a 129/SvJ BAC library (Incyte). A targeting vector was designed using a DNA fragment extending from intron 7 to the 3'UTR, cloned into the pKO scrambler 901 vector (Stratagene). A neomycin cassette flanked by two loxP sites was cloned upstream of exon 16 and a 3rd loxP site was located in exon 8. The exons flanked by loxP sites comprise part of the catalytic domain of RalGDS and residues involved in the binding of the exchange factor to Ras. The construct was electroporated into RW4 ES cells (Incyte) and clones positive for recombination were then transiently transfected with pcrePac vector to eliminate the neomycin cassette. Cells carrying the RalGDS allele lacking exons 9-15 were injected into MF-1 blastocysts and germline-transmitting chimeric mice were obtained. |
| Phenotypic information | Homozygous:No overt phenotype. RalGDS deficiency results in reduced tumor incidence, size, and progression to malignancy in skin carcinogenesis, and reduced transformation by Ras in tissue culture.Heterozygous:No overt phenotype. RalGDS deficiency results in reduced tumor incidence, size, and progression to malignancy in skin carcinogenesis, and reduced transformation by Ras in tissue culture. |
| Breeding history | Animals with a mixed MF-1/129SvJ background were maintained. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- RalGDS is required for tumor formation in a model of skin carcinogenesis.;González-García Ana, Pritchard Catrin A, Paterson Hugh F, Mavria Georgia, Stamp Gordon, Marshall Christopher J, ;2005;Cancer cell;7;219-26; 15766660