MGI phenotypes (allele matching)
B6N.129X1-Scara5tm1Ktry/Kctt
| Status | Available to order |
| EMMA ID | EM:10584 |
| International strain name | B6N.129X1-Scara5tm1Ktry/Kctt |
| Alternative name | B6N.129X1-Scara5 tm1Ktry |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Scara5tm1Ktry, |
| Gene/Transgene symbol | Scara5 |
Information from provider
| Provider | Karl Tryggvason |
| Provider affiliation | Karolinska Institutet |
| Additional owner | Dr. David Lillicrap, Department of Pathology and Molecular Medicine, Richardson Laboratory, Queen’s University, Kingston, Ontario, Canada |
| Genetic information | Most of exon 3 encoding the transmembrane domain of Scara5 was deleted (chromosomal position: 65689781-65689865 bp on chromosome 14) and replaced in-frame by EGFP cassette (EGFP-polyA-Neo, Neomycin gene runs to the opposite direction with its own promoter) with the remaining intracellular sequence of Scara5. |
| Phenotypic information | Homozygous:The mice are born in normal Mendelian distribution without any apparent phenotype. Their lifespan is normal, but with age one can find signs of different autoimmune diseases with inflammatory cell infiltrates in different organs such as lung, skin, liver, adipose tissue. Serum also contains high titres of antinuclear antibodies (ANA). The male mice can develop infertility with age with epididymal obstruction and cell loss in the seminiferous tubuli. This occurs in about 70 % of the male mice and can be seen around 3 months of age.Heterozygous:Normal |
| Breeding history | The mice have been backcrossed more than 10 times with C57BL/6N mice. Currently they have been bred as full knockouts for 2 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | homozygous C57BL/6N |
Disease and phenotype information
Literature references
- Progressive reactive lymphoid connective tissue disease and development of autoantibodies in scavenger receptor A5-deficient mice.;Ojala Juha Risto Matias, Pikkarainen Timo, Elmberger Göran, Tryggvason Karl, ;2013;The American journal of pathology;182;1681-95; 23499552