B6;C-Tg(Spo11-cre)D5Mpel/Cnrm
| Status | Available to order |
| EMMA ID | EM:10645 |
| International strain name | B6;C-Tg(Spo11-cre)D5Mpel/Cnrm |
| Alternative name | Spo11Cre/C57BL/6 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Spo11-cre)D5Mpel |
| Gene/Transgene symbol | Tg(Spo11-cre)D5Mpel |
Information from provider
| Provider | manuela pellegrini |
| Provider affiliation | CNR-IBCN |
| Genetic information | IRES-Cre-Neo fragment of the PL459 plasmid (Liu et al., 2003), flanked by two homology regions of the Spo11 gene immediately downstream from the stop codon site, was electroporated into bacteria containing the Spo11 BAC RP23-20N4. The construct was electroporated in (L)-arabinose inducible EL250 330G11 bacteria for flp-mediated Neo deletion. |
| Phenotypic information | Homozygous:Cre transgene driven by Spo11 regulatory elements is functionally expressed in vivo, in both male and female mice starting from the meiotic stages of gametogenesis. Founders gave germline transmission and the expression of the transgene was identical in several founder lines.Heterozygous:Cre transgene driven by Spo11 regulatory elements is functionally expressed in vivo, in both male and female mice starting from the meiotic stages of gametogenesis. Founders gave germline transmission and the expression of the transgene was identical in several founder lines |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Targeted JAM-C deletion in germ cells by Spo11-controlled Cre recombinase.;Pellegrini Manuela, Claps Giuseppina, Orlova Valeria V, Barrios Florencia, Dolci Susanna, Geremia Raffaele, Rossi Pellegrino, Rossi Gabriele, Arnold Bernd, Chavakis Triantafyllos, Feigenbaum Lionel, Sharan Shyam K, Nussenzweig Andre, ;2011;Journal of cell science;124;91-9; 21147852