C57BL/6-Mir210tm1.1Wtsi/WtsiOulu

Status

Available to order

EMMA IDEM:10855
International strain nameC57BL/6-Mir210tm1.1Wtsi/WtsiOulu
Alternative namemmu-mir-210 knockout
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolMir210tm1.1Wtsi,
Gene/Transgene symbolMir210

Information from provider

ProviderHaydn Prosser
Provider affiliationWellcome Trust Sanger Institute
Genetic informationmmu-mir-210 heterozygous knockout
Phenotypic informationHomozygous:
Mice deficient in miR-210 displayed increased iron-sulfur cluster assembly enzyme (ISCU) 1/2 and were resistant to iron-sulfur (Fe-S) cluster dependent pathophenotypes and pulmonary hypertension.

Heterozygous:
No obvious phenotype recorded.
Breeding historyThe mice were cryopreserved as a mix of C57BL/6Brd-Tyrc-Brd and C57BL/6N. The first generation was bred with albino C57BL/6Brd-Tyrc-Brd in order to establish germline transmission by coat colour. They were then bred onto C57BL/6N (CBLN or CBLT) for five generations, which is the way genetic background was historically standardized before the widespread use of C57BL/6N ES cells. Unfortunately we then almost lost the line as a generation did not breed and Sanger Team 121 did an emergency IVF, but in doing so they used an N2 backcrossed male in addition to an N5 backcrossed male and mixed the sperm so that the IVF offspring could be N3 or N6 backcrossed. The line is cryopreserved at the archiving centre in Oulu, Finland.
References
  • Genetic and hypoxic alterations of the microRNA-210-ISCU1/2 axis promote iron-sulfur deficiency and pulmonary hypertension.;White Kevin, Lu Yu, Annis Sofia, Hale Andrew E, Chau B Nelson, Dahlman James E, Hemann Craig, Opotowsky Alexander R, Vargas Sara O, Rosas Ivan, Perrella Mark A, Osorio Juan C, Haley Kathleen J, Graham Brian B, Kumar Rahul, Saggar Rajan, Saggar Rajeev, Wallace W Dean, Ross David J, Khan Omar F, Bader Andrew, Gochuico Bernadette R, Matar Majed, Polach Kevin, Johannessen Nicolai M, Prosser Haydn M, Anderson Daniel G, Langer Robert, Zweier Jay L, Bindoff Laurence A, Systrom David, Waxman Aaron B, Jin Richard C, Chan Stephen Y, ;2015;EMBO molecular medicine;7;695-713; 25825391
  • MiR-210 is induced by Oct-2, regulates B cells, and inhibits autoantibody production.;Mok Yingting, Schwierzeck Vera, Thomas David C, Vigorito Elena, Rayner Tim F, Jarvis Lorna B, Prosser Haydn M, Bradley Allan, Withers David R, Mårtensson Inga-Lill, Corcoran Lynn M, Blenkiron Cherie, Miska Eric A, Lyons Paul A, Smith Kenneth G C, ;2013;Journal of immunology (Baltimore, Md. : 1950);191;3037-3048; 23960236
Homozygous fertileyes
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Oulu, Oulu, Finland

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased anti-double stranded DNA antibody level / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • increased anti-chromatin antibody level / MGI
  • immune system phenotype / MGI
  • decreased germinal center B cell number / MGI

Literature references

  • Genetic and hypoxic alterations of the microRNA-210-ISCU1/2 axis promote iron-sulfur deficiency and pulmonary hypertension.;White Kevin, Lu Yu, Annis Sofia, Hale Andrew E, Chau B Nelson, Dahlman James E, Hemann Craig, Opotowsky Alexander R, Vargas Sara O, Rosas Ivan, Perrella Mark A, Osorio Juan C, Haley Kathleen J, Graham Brian B, Kumar Rahul, Saggar Rajan, Saggar Rajeev, Wallace W Dean, Ross David J, Khan Omar F, Bader Andrew, Gochuico Bernadette R, Matar Majed, Polach Kevin, Johannessen Nicolai M, Prosser Haydn M, Anderson Daniel G, Langer Robert, Zweier Jay L, Bindoff Laurence A, Systrom David, Waxman Aaron B, Jin Richard C, Chan Stephen Y, ;2015;EMBO molecular medicine;7;695-713; 25825391
  • MiR-210 is induced by Oct-2, regulates B cells, and inhibits autoantibody production.;Mok Yingting, Schwierzeck Vera, Thomas David C, Vigorito Elena, Rayner Tim F, Jarvis Lorna B, Prosser Haydn M, Bradley Allan, Withers David R, Mårtensson Inga-Lill, Corcoran Lynn M, Blenkiron Cherie, Miska Eric A, Lyons Paul A, Smith Kenneth G C, ;2013;Journal of immunology (Baltimore, Md. : 1950);191;3037-3048; 23960236

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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