B6.Cg-Setdb2tm1Desr/Biat

Status

Available to order

EMMA IDEM:11045
International strain nameB6.Cg-Setdb2tm1Desr/Biat
Alternative nameSetdb2_ex3GT/GT
Strain typeGene-trap
Allele/Transgene symbolSetdb2tm1Desr,
Gene/Transgene symbolSetdb2

Information from provider

ProviderDavid Symer
Provider affiliationDept. of Lymphoma & Myeloma, Univ. Texas MD Anderson Cancer Center
Genetic informationTo generate Setdb2GT/GT mice, the targeting vector pEKF106 was made by recombination using Lambda RED system in E. coli strain EL35047. A genomic PAC library (i.e. from the 129/SvEvTac fBr genetic background, RPCI mouse PAC library 21; MRC Geneservice) was screened for the full-length Setdb2 gene using specific cDNA probes. A positive PAC clone, RP21-498J23, was used for recovery of flanking genomic sequences. We generated a 2.3kb genomic fragment introducing an EcoRV restriction site and loxP-neo using plasmid pLMJ237, for insertion downstream of exon 3. Neo was removed by arabinose induction of Cre recombinase. Similarly, a 2.9kb genomic fragment harbouring the frt-loxP flanked gene trap cassette and a heterologous BamHI restriction site was generated by recombination using pLTM330. The gene trap cassette contains a pGK/EM7 dual promoter, a strong splice acceptor site from the engrailed 2 gene and Neo reporter gene, followed by a strong polyadenylation signal (Supplementary Fig. 3A). NotI linearized targeting vector DNA was transfected into v6.4 embryonic stem (ES) cells, a hybrid of the C57BL/6J × 129/SvJae lineages. Transfected ES cells were selected for neomycin resistance with G418 and positive clones were tested by Southern blot analysis of EcoRV-digested DNA. Positive ES cell clones were microinjected into blastulae and transferred to pseudopregnant female mice, following standard methods.
Phenotypic informationHomozygous:
altered immune response

Heterozygous:
unknown
References
  • The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection.;Schliehe Christopher, Flynn Elizabeth K, Vilagos Bojan, Richson Udochuku, Swaminanthan Savitha, Bosnjak Berislav, Bauer Lisa, Kandasamy Richard K, Griesshammer Isabel M, Kosack Lindsay, Schmitz Frank, Litvak Vladimir, Sissons James, Lercher Alexander, Bhattacharya Anannya, Khamina Kseniya, Trivett Anna L, Tessarollo Lino, Mesteri Ildiko, Hladik Anastasiya, Merkler Doron, Kubicek Stefan, Knapp Sylvia, Epstein Michelle M, Symer David E, Aderem Alan, Bergthaler Andreas, ;2015;Nature immunology;16;67-74; 25419628
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased neutrophil cell number / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal response to infection / MGI

Literature references

  • The methyltransferase Setdb2 mediates virus-induced susceptibility to bacterial superinfection.;Schliehe Christopher, Flynn Elizabeth K, Vilagos Bojan, Richson Udochuku, Swaminanthan Savitha, Bosnjak Berislav, Bauer Lisa, Kandasamy Richard K, Griesshammer Isabel M, Kosack Lindsay, Schmitz Frank, Litvak Vladimir, Sissons James, Lercher Alexander, Bhattacharya Anannya, Khamina Kseniya, Trivett Anna L, Tessarollo Lino, Mesteri Ildiko, Hladik Anastasiya, Merkler Doron, Kubicek Stefan, Knapp Sylvia, Epstein Michelle M, Symer David E, Aderem Alan, Bergthaler Andreas, ;2015;Nature immunology;16;67-74; 25419628

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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