MGI phenotypes (allele matching)
B6.129S-Tnfrsf1atm1Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:11071 |
| International strain name | B6.129S-Tnfrsf1atm1Gkl/Flmg |
| Alternative name | B6.Cg-Tnfrsf1a |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Tnfrsf1atm1Gkl, |
| Gene/Transgene symbol | Tnfrsf1a |
Information from provider
| Provider | George Kollias |
| Provider affiliation | BSRC Fleming |
| Genetic information | A mutation deleting 15 nucleotides encoding amino acids 202-206 was introduced in exon 6 of mp55TNFR (Tnfrsf1a) gene. The targeting vector contained a 4.6-kb XbaI/SmaI genomic fragment as the left homology arm and a 4-kb Sma/HindIII genomic fragment, containing the mutation, as the right homology arm. A loxP flanked neo selection cassette (L2neo) was introduced into a SmaI site in intron 5 of Tnfrsf1a gene between the two homology arms and a PGK-tk selection marker was introduced at the 3' of targeting vector. Upon cre-mediated recombination, a cell-specific Tnfrsf1a-tm1.1Gkl allele is generated. |
| Phenotypic information | Homozygous:No overt phenotype. Homozygous mice are fertile but they are smaller than their littermates and in poor health so we do not mate the homozygous mice. Heterozygous:No overt phenotype |
| Breeding history | Homozygous mice and wild-type controls are generated by crossing heterozygous mice. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | homozygous C57BL/6, wild-type C57BL/6 |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960
Literature references
- Tumor necrosis factor (TNF) receptor shedding controls thresholds of innate immune activation that balance opposing TNF functions in infectious and inflammatory diseases.;Xanthoulea Sofia, Pasparakis Manolis, Kousteni Stavroula, Brakebusch Cord, Wallach David, Bauer Jan, Lassmann Hans, Kollias George, ;2004;The Journal of experimental medicine;200;367-76; 15289505