B6.129S7-Tbx1tm6(cre)Bld/Cnrm

Status

Available to order

EMMA IDEM:11399
International strain nameB6.129S7-Tbx1tm6(cre)Bld/Cnrm
Alternative nameTbx1Cre
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolTbx1tm6(cre)Bld,
Gene/Transgene symbolTbx1

Information from provider

ProviderAntonio Baldini
Provider affiliationIstituto di Genetica e Biofisica CNR
Genetic informationThe allele was generated by knocking in a cre-encoding sequence into exon 5 (9-exons gene model) of the Tbx1 gene using homologous recombination in ES cells (AB2.2). Described in the publication: A fate map of Tbx1 expressing cells reveals heterogeneity in the second cardiac field. Huynh T, Chen L, Terrell P, Baldini A. Genesis. 2007 Jul;45(7):470-5.
Phenotypic informationHomozygous:
Homozygous animals die at birth because of lethal heart defects and other congenital anomalies. The cre allele is null, therefore, the phenotype is identical to the one described in the literature for Tbx1 mutants, e.g.: Vitelli F, Morishima M, Taddei I, Lindsay EA, Baldini A. Hum. Mol. Genet. 11:915-922, 2002.

Heterozygous:
Cre knock-in inactivates the Tbx1 gene. Tbx1 is haploinsufficient. Heterozygous animals have defects including aortic arch remodelling. Depending upon genetic background, the heterozygous mutation may cause perinatal lethality in 3-6% of the animals.
Breeding historyThe stock of this line is maintained at the heterozygous state by crossing males with C57BL/6 females, in a conventional clean facility.
References
  • A fate map of Tbx1 expressing cells reveals heterogeneity in the second cardiac field.;Huynh Tuong, Chen Li, Terrell Phillip, Baldini Antonio, ;2007;Genesis (New York, N.Y. : 2000);45;470-5; 17610275
  • A phenotypic rescue approach identifies lineage regionalization defects in a mouse model of DiGeorge syndrome.;Lania Gabriella, Franzese Monica, Adachi Noritaka, Bilio Marchesa, Flore Gemma, Russo Annalaura, D'Agostino Erika, Angelini Claudia, Kelly Robert G, Baldini Antonio, ;2022;Disease models & mechanisms;15;; 35946435
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • persistent truncus arteriosis / MGI
  • ventricular septal defect / MGI
  • abnormal blood vessel morphology / MGI

Literature references

  • A fate map of Tbx1 expressing cells reveals heterogeneity in the second cardiac field.;Huynh Tuong, Chen Li, Terrell Phillip, Baldini Antonio, ;2007;Genesis (New York, N.Y. : 2000);45;470-5; 17610275
  • A phenotypic rescue approach identifies lineage regionalization defects in a mouse model of DiGeorge syndrome.;Lania Gabriella, Franzese Monica, Adachi Noritaka, Bilio Marchesa, Flore Gemma, Russo Annalaura, D'Agostino Erika, Angelini Claudia, Kelly Robert G, Baldini Antonio, ;2022;Disease models & mechanisms;15;; 35946435

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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