STOCK Gt(ROSA)26Sortm1(CAG-ChR2/tdTomato)Oki/Kctt
| Status | Available to order |
| EMMA ID | EM:11486 |
| International strain name | STOCK Gt(ROSA)26Sortm1(CAG-ChR2/tdTomato)Oki/Kctt |
| Alternative name | ROSA26-CAG-frt-STOP-frt-loxp-NLS/LacZ-loxP-ChR2-tdT |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(CAG-ChR2/tdTomato)Oki |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Ole Kiehn |
| Provider affiliation | Neuroscience, Karolinska Institutet |
| Genetic information | This RC::FLTG dual recombinase reporter construct contains a frt-flanked STOP cassette followed by a loxP-flanked NLS beta-gal::STOP cassette in front of ChR2-tdTomato. The ChR2-tdTomato contains a p2A self-cleaving peptide sequence between ChR2 and tdTomato, which leads to the expression of ChR2 and tdTomato as two separate proteins. These mice allow intersectional genetic optogenetic stimulation of different cell subpopulations defined by the overlap of FlipO and cre recombinase expression and can simultaneously be used for intersectional fluorescent labelling of cells since tdTomato is not bound to the membrane. |
| Phenotypic information | Homozygous:No visible phenotype. Homozygous mice are fertile and behave as wildtype mice.Heterozygous:No visible phenotype. Heterozygous males behave like wildtype mice. |
| Breeding history | The strain has been backcrossed with C57BL/6J for 4 generations and kept heterozygous. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J |