STOCK Nox4tm1.2Hwsc Tg(Camk2a-cre)2Gsc/Orl
Status | Available to order |
EMMA ID | EM:11554 |
International strain name | STOCK Nox4tm1.2Hwsc Tg(Camk2a-cre)2Gsc/Orl |
Alternative name | C57BL/6-Tg(Camk2a-cre)2Gsc(Nox4-FF) |
Strain type | Targeted Mutant Strains : Conditional mutation |
Allele/Transgene symbol | Nox4tm1.2Hwsc, |
Gene/Transgene symbol | Nox4 |
Information from provider
Provider | Harald Schmidt |
Provider affiliation | Department of Pharmacology & Personalised Medicine, Maastricht University |
Genetic information | The Nox4 gene is flanked by cre-lox sites around exons 14 and 15; cre-loxP technology, type 1 mutation for conditional knock-outs. These mice were crossed with Camk2a-cre (CamKIICre) mice to get specific Nox4 deletion in neuronal cells. |
Phenotypic information | Homozygous:No spontaneous phenotype. Decreased infarct size in strokeHeterozygous:No spontaneous phenotype. |
Breeding history | To generate neuron-specific Nox4 knock-out (nNOX4 KO) mice, female mice (homozygous for the floxed Nox4 gene) were bred with male mice (C57BL/6 strain background) that express the cre recombinase gene under control of the neuronal-cell specific Camk2a (CamKIIalpha) promotor. As the cre is located on the X chromosome, all males used for this breeding were hemizygous for the cre gene (cre+/y). During breeding, only males that bear the cre gene were selected for future breeding rounds, while females were not allowed to bear the cre. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Animals used for archiving | heterozygous C57BL/6N, wild-type C57BL/6N |
Stage of embryos | 2-cell |
Literature references
- NOX4-dependent neuronal autotoxicity and BBB breakdown explain the superior sensitivity of the brain to ischemic damage.;Casas Ana I, Geuss Eva, Kleikers Pamela W M, Mencl Stine, Herrmann Alexander M, Buendia Izaskun, Egea Javier, Meuth Sven G, Lopez Manuela G, Kleinschnitz Christoph, Schmidt Harald H H W, ;2017;Proceedings of the National Academy of Sciences of the United States of America;114;12315-12320; 29087944
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