B6;Cg-H2axtm1Nus/Cnrm
Status | Available to order |
EMMA ID | EM:11818 |
International strain name | B6;Cg-H2axtm1Nus/Cnrm |
Alternative name | H2afx+/- |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | H2axtm1Nus, |
Gene/Transgene symbol | H2ax |
Information from provider
Provider | Andre Nussenzweig |
Provider affiliation | Center for Cancer Research, National Cancer Institute |
Genetic information | A genomic clone covering the H2ax locus was isolated from a 129/Sv mouse lambda phage library (Stratagene). The targeting construct was designed as a null mutation that replaces a segment of H2ax between the unique Asc1 and Xho1 site in the coding sequence by a neomycin resistance cassette. |
Phenotypic information | Homozygous:H2ax-/- mice were radiation sensitive, growth retarded, and immune deficient, and mutant males were infertile.Heterozygous:Phenotype of het. mice was similar to wild-type controls. |
References |
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Homozygous fertile | females only |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Genomic instability in mice lacking histone H2AX.;Celeste Arkady, Petersen Simone, Romanienko Peter J, Fernandez-Capetillo Oscar, Chen Hua Tang, Sedelnikova Olga A, Reina-San-Martin Bernardo, Coppola Vincenzo, Meffre Eric, Difilippantonio Michael J, Redon Christophe, Pilch Duane R, Olaru Alexandru, Eckhaus Michael, Camerini-Otero R Daniel, Tessarollo Lino, Livak Ferenc, Manova Katia, Bonner William M, Nussenzweig Michel C, Nussenzweig André, ;2002;Science (New York, N.Y.);296;922-7; 11934988
- AID is required to initiate Nbs1/gamma-H2AX focus formation and mutations at sites of class switching.;Petersen Simone, Casellas Rafael, Reina-San-Martin Bernardo, Chen Hua Tang, Difilippantonio Michael J, Wilson Patrick C, Hanitsch Leif, Celeste Arkady, Muramatsuk Masamichi, Pilch Duane R, Redon Christophe, Ried Thomas, Bonner William M, Honjo Tasuku, Nussenzweig Michel C, Nussenzweig André, ;2001;Nature;414;660-665; 11740565
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