Rosa26-tidICKD-UMCM line 2
| Status | Available to order |
| EMMA ID | EM:11917 |
| International strain name | Rosa26-tidICKD-UMCM line 2 |
| Alternative name | Rosa26-tidICKD-UMCM line 2 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gt(ROSA)26Sortm(Dnaja3-TidIC) |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Ursula Kurzik-Dumke |
| Provider affiliation | Institut für Medizinische Mikrobiologie und Hygiene, Johannes Gutenberg-Universität Mainz |
| Genetic information | Conditional knock-in of the Tid (Dnaja3) IC splice form (GenBank Acc. Nr.: AF325535; PubMed ID:11116152) into Gt(ROSA)26Sor, homozygous. The cDNA encoding the Tid splice form was cloned into the pRAPIDflirt vector. The structure of the targeting vector is as follows: Gt(ROSA)26Sor::CAG–NeoR–WSS/STOP–Tid splice form-IRES–EGFP. The NeoR-WSS/STOP cassette is floxed. Thus, the activation of Tid expression only occurs after cre-mediated excision of the cassette. Because of the EGFP-coding sequence the mutant mice express it, too. To generate the knock-in mutant line Bruce 4 ES cells (C57BL/6-derived) were used. Recombinant ES cells were injected into C57BL/6 albino blastocysts. For GLT chimeric males were mated with C57BL/6 albino females. |
| Phenotypic information | Homozygous:wild-typeHeterozygous:wild-type |
| Breeding history | 14 inbred generations |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous C57BL/6J |