B6.FVB-Tg(Slc1a3-cre/ERT2)45-72Fwp/Orl

Status

Available to order

EMMA IDEM:12216
International strain nameB6.FVB-Tg(Slc1a3-cre/ERT2)45-72Fwp/Orl
Alternative nameTg(Slc1a3-cre/ERT2)45-72Fwp
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Slc1a3-cre/ERT2)45-72Fwp,
Gene/Transgene symbolTg(Slc1a3-cre/ERT2)45-72Fwp

Information from provider

ProviderFrank Pfrieger
Provider affiliationInstitute of Cellular and Integrative Neurosciences CNRS UPR 3212
Additional ownerProf. Pierre Chambon, IGBMC, Illkirch, France
Genetic informationThe creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Slc1a3 (Glast) gene located in BAC RP23-63O21. The linearized vector was injected into pronuclei of fertilized FVB/N eggs.
Phenotypic informationHomozygous:
Transgenic line for inducible somatic mutagenesis in Slc1a3/Glast-expressing cells, notably astroglial cells throughout the brain, adult neural cells/progenitors in neurogenic regions and retinal astroglial cells of the retina.

Heterozygous:
Transgenic line for inducible somatic mutagenesis in Slc1a3/Glast-expressing cells, notably astroglial cells throughout the brain, adult neural cells/progenitors in neurogenic regions and retinal astroglial cells of the retina.
Breeding historyBackcrossed on C57BL/6 for more than 10 generations
References
  • Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
  • Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
  • RBPJkappa-dependent signaling is essential for long-term maintenance of neural stem cells in the adult hippocampus.;Ehm Oliver, Göritz Christian, Covic Marcela, Schäffner Iris, Schwarz Tobias J, Karaca Esra, Kempkes Bettina, Kremmer Elisabeth, Pfrieger Frank W, Espinosa Lluis, Bigas Anna, Giachino Claudio, Taylor Verdon, Frisén Jonas, Lie D Chichung, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;13794-807; 20943920
  • A pericyte origin of spinal cord scar tissue.;Göritz Christian, Dias David O, Tomilin Nikolay, Barbacid Mariano, Shupliakov Oleg, Frisén Jonas, ;2011;Science (New York, N.Y.);333;238-42; 21737741
  • The Role of Hypothalamic NF-κB Signaling in the Response of the HPT-Axis to Acute Inflammation in Female Mice.;de Vries E M, Nagel S, Haenold R, Sundaram S M, Pfrieger F W, Fliers E, Heuer H, Boelen A, ;2016;Endocrinology;157;2947-56; 27187176
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6J

Literature references

  • Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
  • Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
  • RBPJkappa-dependent signaling is essential for long-term maintenance of neural stem cells in the adult hippocampus.;Ehm Oliver, Göritz Christian, Covic Marcela, Schäffner Iris, Schwarz Tobias J, Karaca Esra, Kempkes Bettina, Kremmer Elisabeth, Pfrieger Frank W, Espinosa Lluis, Bigas Anna, Giachino Claudio, Taylor Verdon, Frisén Jonas, Lie D Chichung, ;2010;The Journal of neuroscience : the official journal of the Society for Neuroscience;30;13794-807; 20943920
  • A pericyte origin of spinal cord scar tissue.;Göritz Christian, Dias David O, Tomilin Nikolay, Barbacid Mariano, Shupliakov Oleg, Frisén Jonas, ;2011;Science (New York, N.Y.);333;238-42; 21737741
  • The Role of Hypothalamic NF-κB Signaling in the Response of the HPT-Axis to Acute Inflammation in Female Mice.;de Vries E M, Nagel S, Haenold R, Sundaram S M, Pfrieger F W, Fliers E, Heuer H, Boelen A, ;2016;Endocrinology;157;2947-56; 27187176

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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