B6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl

Status

Available to order

EMMA IDEM:12217
International strain nameB6.FVB-Tg(Gjb6-cre/ERT2)53-33Fwp/Orl
Alternative nameTg(Gjb6-cre/ERT2)53-33Fwp
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Gjb6-cre/ERT2)53-33Fwp,
Gene/Transgene symbolTg(Gjb6-cre/ERT2)53-33Fwp

Information from provider

ProviderFrank Pfrieger
Provider affiliationInstitute of Cellular and Integrative Neurosciences CNRS UPR 3212
Additional ownerProf. Pierre Chambon, IGBMC, Illkirch, France
Genetic informationThe creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Gjb6 gene located in BAC RP24-368M7. The linearized vector was injected into pronuclei of fertilized FVB/N eggs.
Phenotypic informationHomozygous:
The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system.

Heterozygous:
The line allows for inducible somatic mutagenesis in Gjb6-/Cx30-expressing cells, notably astrocytes in different regions of the central nervous system.
Breeding historyBackcrossed to C57BL/6 for more than 10 generations
References
  • Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
  • Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
  • Role of astroglial connexin30 in hippocampal gap junction coupling.;Gosejacob Dominic, Dublin Pavel, Bedner Peter, Hüttmann Kerstin, Zhang Jiong, Tress Oliver, Willecke Klaus, Pfrieger Frank, Steinhäuser Christian, Theis Martin, ;2011;Glia;59;511-9; 21264956
  • Calcium dynamics in astrocyte processes during neurovascular coupling.;Otsu Yo, Couchman Kiri, Lyons Declan G, Collot Mayeul, Agarwal Amit, Mallet Jean-Maurice, Pfrieger Frank W, Bergles Dwight E, Charpak Serge, ;2015;Nature neuroscience;18;210-8; 25531572
  • An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.;Shen Weida, Nikolic Ljiljana, Meunier Claire, Pfrieger Frank, Audinat Etienne, ;2017;Scientific reports;7;11280; 28900295
  • Glucocorticoid receptor in astrocytes regulates midbrain dopamine neurodegeneration through connexin hemichannel activity.;Maatouk Layal, Yi Chenju, Carrillo-de Sauvage Maria-Angeles, Compagnion Anne-Claire, Hunot Stéphane, Ezan Pascal, Hirsch Etienne C, Koulakoff Annette, Pfrieger Frank W, Tronche François, Leybaert Luc, Giaume Christian, Vyas Sheela, ;2019;Cell death and differentiation;26;580-596; 30006609
  • Astrocyte-derived Jagged-1 mitigates deleterious Notch signaling in amyotrophic lateral sclerosis.;Nonneman Annelies, Criem Nathan, Lewandowski Sebastian A, Nuyts Rik, Thal Dietmar R, Pfrieger Frank W, Ravits John, Van Damme Philip, Zwijsen An, Van Den Bosch Ludo, Robberecht Wim, ;2018;Neurobiology of disease;119;26-40; 30010003
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Literature references

  • Transgenic mice for conditional gene manipulation in astroglial cells.;Slezak Michal, Göritz Christian, Niemiec Aurore, Frisén Jonas, Chambon Pierre, Metzger Daniel, Pfrieger Frank W, ;2007;Glia;55;1565-76; 17823970
  • Origin of new glial cells in intact and injured adult spinal cord.;Barnabé-Heider Fanie, Göritz Christian, Sabelström Hanna, Takebayashi Hirohide, Pfrieger Frank W, Meletis Konstantinos, Frisén Jonas, ;2010;Cell stem cell;7;470-82; 20887953
  • Role of astroglial connexin30 in hippocampal gap junction coupling.;Gosejacob Dominic, Dublin Pavel, Bedner Peter, Hüttmann Kerstin, Zhang Jiong, Tress Oliver, Willecke Klaus, Pfrieger Frank, Steinhäuser Christian, Theis Martin, ;2011;Glia;59;511-9; 21264956
  • Calcium dynamics in astrocyte processes during neurovascular coupling.;Otsu Yo, Couchman Kiri, Lyons Declan G, Collot Mayeul, Agarwal Amit, Mallet Jean-Maurice, Pfrieger Frank W, Bergles Dwight E, Charpak Serge, ;2015;Nature neuroscience;18;210-8; 25531572
  • An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.;Shen Weida, Nikolic Ljiljana, Meunier Claire, Pfrieger Frank, Audinat Etienne, ;2017;Scientific reports;7;11280; 28900295
  • Glucocorticoid receptor in astrocytes regulates midbrain dopamine neurodegeneration through connexin hemichannel activity.;Maatouk Layal, Yi Chenju, Carrillo-de Sauvage Maria-Angeles, Compagnion Anne-Claire, Hunot Stéphane, Ezan Pascal, Hirsch Etienne C, Koulakoff Annette, Pfrieger Frank W, Tronche François, Leybaert Luc, Giaume Christian, Vyas Sheela, ;2019;Cell death and differentiation;26;580-596; 30006609
  • Astrocyte-derived Jagged-1 mitigates deleterious Notch signaling in amyotrophic lateral sclerosis.;Nonneman Annelies, Criem Nathan, Lewandowski Sebastian A, Nuyts Rik, Thal Dietmar R, Pfrieger Frank W, Ravits John, Van Damme Philip, Zwijsen An, Van Den Bosch Ludo, Robberecht Wim, ;2018;Neurobiology of disease;119;26-40; 30010003

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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