C57BL/6N-Tg(Vav1-STAT5B*N642H)726Biat/Biat

Status	Available to order
EMMA ID	EM:12475
International strain name	C57BL/6N-Tg(Vav1-STAT5B*N642H)726Biat/Biat
Alternative name	B6N-Tg(STAT5BN/H)726Biat
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Vav1-STAT5B*N642H)726Biat
Gene/Transgene symbol	Tg(Vav1-STAT5B*N642H)726Biat

Information from provider

Provider	Richard Moriggl
Provider affiliation	Department for Biomedical Sciences, University of Veterinary Medicine, Vienna (Vetmeduni Vienna)
Genetic information	Transgenic mice were generated and bred on a C57BL/6NCrl background. We used the Vav1-hematopoietic vector Vav1-hCD4 (HS21/45) to generate several transgenic mouse lines expressing hSTAT5B and hSTAT5BN642H in the hematopoietic system and selected the lines B6N-Tg(STAT5B)731Biat (EMMA ID EM:12476) and B6N-Tg(STAT5BN/H)726Biat (this one; EMMA ID EM:12475), respectively, for further experiments. The hSTAT5BN642H construct was generated using overlapping PCR technology.
Phenotypic information	Homozygous: Unknown; homozygous were not produced. Heterozygous: hSTAT5BN642H mice develop an aggressive CD8+ T cell lymphoma at the age of 6-12 weeks. This goes in line with a massive expansion of spleen and lymphonodes, as well as organ infiltrations. For further details see Pham HTT et al., 2018. The heterozygous B6N Tg(STAT5B)731Biat line (EMMA ID EM:12476) exhibits no obvious phenotype and is used as an experimental control.
Breeding history	Less than 10 generations of backcross to C57BL/6N.
References	STAT5BN642H is a driver mutation for T cell neoplasia.;Pham Ha Thi Thanh, Maurer Barbara, Prchal-Murphy Michaela, Grausenburger Reinhard, Grundschober Eva, Javaheri Tahereh, Nivarthi Harini, Boersma Auke, Kolbe Thomas, Elabd Mohamed, Halbritter Florian, Pencik Jan, Kazemi Zahra, Grebien Florian, Hengstschläger Markus, Kenner Lukas, Kubicek Stefan, Farlik Matthias, Bock Christoph, Valent Peter, Müller Mathias, Rülicke Thomas, Sexl Veronika, Moriggl Richard, ;2018;The Journal of clinical investigation;128;387-401; 29200404
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Animals used for archiving	heterozygous C57BL/6N

Disease and phenotype information

MGI allele-associated human disease models

leukemia / DOID:1240
lymphoma / DOID:0060058

Literature references

STAT5BN642H is a driver mutation for T cell neoplasia.;Pham Ha Thi Thanh, Maurer Barbara, Prchal-Murphy Michaela, Grausenburger Reinhard, Grundschober Eva, Javaheri Tahereh, Nivarthi Harini, Boersma Auke, Kolbe Thomas, Elabd Mohamed, Halbritter Florian, Pencik Jan, Kazemi Zahra, Grebien Florian, Hengstschläger Markus, Kenner Lukas, Kubicek Stefan, Farlik Matthias, Bock Christoph, Valent Peter, Müller Mathias, Rülicke Thomas, Sexl Veronika, Moriggl Richard, ;2018;The Journal of clinical investigation;128;387-401; 29200404

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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Practical information

Example health report
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