C3HeB/FeJ-CrygdAey4/Ieg

Status

Available to order

EMMA IDEM:12520
International strain nameC3HeB/FeJ-CrygdAey4/Ieg
Alternative nameCrygd-Aey4
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolCrygdAey4,
Gene/Transgene symbolCrygd

Information from provider

ProviderMartin Hrabé de Angelis
Provider affiliationInstitute of Experimental Genetics, Helmholtz Center Munich
Additional ownerProf. Dr. Jochen Graw, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Institute of Developmental Genetics, Neuherberg/Germany
Genetic informationA T to A exchange in exon 2 of the crystallin, gamma D encoding gene (symbol: Crygd) was demonstrated to be causative for the cataract phenotype; this particular mutation is, therefore, referred to as Crygd-Aey4. The alteration in codon 76 leads to an amino acid exchange of Val to Asp (V76D).

Disclaimer - Special restrictions:

1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols.
2) Some lines showed low penetrance of the phenotype.
3) Only sperm available. Rederivation service can not be offered.
4) EMMA quality control standards may not apply for these strains.
5) EMMA has not verified the breeding performance and the genetic background of the strains.

The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic informationHomozygous:
The slit lamp examination revealed a strong nuclear cataract surrounded by a homogenous milky opacity in the inner cortex. The histological analysis demonstrated remnants of cell nuclei throughout the entire lens.

Heterozygous:
The slit lamp examination revealed a strong nuclear cataract surrounded by a homogenous milky opacity in the inner cortex. The histological analysis demonstrated remnants of cell nuclei throughout the entire lens.
Breeding historyThe mutants were kept as a homozygous line since its re-derivation from frozen sperms since 2014.
References
  • V76D mutation in a conserved gD-crystallin region leads to dominant cataracts in mice.;Graw Jochen, Löster Jana, Soewarto Dian, Fuchs Helmut, Reis André, Wolf Eckhard, Balling Rudi, Hrabé de Angelis Martin, ;2002;Mammalian genome : official journal of the International Mammalian Genome Society;13;452-5; 12226711
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivinghomozygous C3HeB/FeJ

Disease and phenotype information

MGI allele-associated human disease models

Literature references

  • V76D mutation in a conserved gD-crystallin region leads to dominant cataracts in mice.;Graw Jochen, Löster Jana, Soewarto Dian, Fuchs Helmut, Reis André, Wolf Eckhard, Balling Rudi, Hrabé de Angelis Martin, ;2002;Mammalian genome : official journal of the International Mammalian Genome Society;13;452-5; 12226711

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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