129S6;B6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze Tg(Gad2-Ncre)Jhi Tg(Slc6a5-Ccre)Jhi/Ph
| Status | Available to order |
| EMMA ID | EM:12607 |
| International strain name | 129S6;B6-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze Tg(Gad2-Ncre)Jhi Tg(Slc6a5-Ccre)Jhi/Ph |
| Alternative name | COTRANS [Gad2-NCre x Slc6a5-CCre x Ai14] |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gad2-Ncre)1Jhi |
| Gene/Transgene symbol | Tg(Gad2-Ncre)1Jhi |
Information from provider
| Provider | Johannes Hirrlinger |
| Provider affiliation | Carl-Ludwig-Institute for Physiology, University of Leipzig |
| Additional owner | Hongkui Zheng, PhD, Allen Institute for Brain Science, Seattle, USA |
| Genetic information | This is a triple transgenic mouse line allowing to genetically target neurons which use GABA and glycine at the same time as neurotransmitters, based on cre recombinase-mediated DNA recombination using the split-cre system (Hirrlinger et al 2009; doi: 10.1371/journal.pone.0004286). It contains the following three transgenic alleles: 1) BAC[Gad2-NCre-IRES-beta galactosidase-SV40pA] in which the NCre-IRES-beta galactosidase-SV40pA sequence was inserted by homologous recombination into BAC clone 407K8 at the ATG initiation codon of exon 1 of the Gad2 gene. 2) BAC[Slc6a5-CCre-IRES-alkaline phosphatase-SV40pA] in which the CCre-IRES-alkaline phosphatase-SV40pA sequence was inserted by homologous recombination into exon 2 of the Slc6a5 gene (also known as glycine transporter 2, GlyT2) within BAC clone 365E4 using homologous recombination. 3) The Gt(ROSA)26Sortm14(CAG-tdTomato)Hze (Ai14) reporter allele, expressing tdTomato after cre recombinase-mediated DNA recombination (Madisen et al 2010, DOI: 10.1038/nn.2467). |
| Phenotypic information | Homozygous:The mice do not show any general phenotype. However, neurons which at any time in their life use both GABA and glycine at the same time as neurotransmitter (i.e. GABA-glycine co-transmitting neurons) are labelled irreversibly due to tdTomato expression from the Ai14 reporter allele, induced by cre recombinase/loxP-mediated DNA recombination. Besides its use to track co-transmitting neurons by labelling via reporter gene expression, this mouse line can be combined with any mouse line harboring a loxP-flanked gene to delete it in co-transmitting neurons. Furthermore, each single transgene (Gad2-NCre as well as Slc6a5-CCre) can be used on its own (after appropriate crossings to remove the other transgene) to be combined with any other split-cre based transgene to change the cell type specificity of recombination.Heterozygous:Same as for homozygous mice. |
| Breeding history | Transgenic strains BAC[Gad2-NCre-IRES- betaGalactosidase-SV40pA] and BAC[Slc6a5-CCre-IRES- alkaline phosphatase-SV40pA] were generated individually by oocyte injections of the BAC constructs. Lines were then crossed with each other as well as with the Ai14 reporter line. In most animals, the Ai14 reporter allele is maintained homozygous, while the two BAC-based split-cre recombinase alleles are heterozygous. The BAC constructs were initially injected into oocytes of C57BL/6N. In the further course, however, they were paired with C57BL/6J. The reporter mouse line Ai14 was obtained from another lab in a C57BL/6 background (not specified if B6J or B6N). |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Stage of embryos | 2-cell |
Literature references
- GABA-Glycine Cotransmitting Neurons in the Ventrolateral Medulla: Development and Functional Relevance for Breathing.;Hirrlinger Johannes, Marx Grit, Besser Stefanie, Sicker Marit, Köhler Susanne, Hirrlinger Petra G, Wojcik Sonja M, Eulenburg Volker, Winkler Ulrike, Hülsmann Swen, ;2019;Frontiers in cellular neuroscience;13;517; 31803026