B6.129-Plxnb1tm1.1Ltam/Cnrm

Status

Available to order

EMMA IDEM:12800
International strain nameB6.129-Plxnb1tm1.1Ltam/Cnrm
Alternative namePlxnB1-KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPlxnb1tm1.1Ltam,
Gene/Transgene symbolPlxnb1

Information from provider

ProviderLuca Tamagnone
Provider affiliationIstituto di Istologia ed Embriologia, Università Cattolica del Sacro Cuore
Genetic informationPlexin-B1 gene inactivation by insertion of loxP sites, and subsequent deletion of exons 22-23.
Phenotypic informationHomozygous:
They do not show any major developmental phenotype, apart from a kidney phenotype appearing at E13.5 (kidneys are larger and exhibit more ureteric branches than in heterozygous controls; Perala et al., 2011). Due to important role of plexin-B1 signaling reported in the immune system and cancer microenvironment, further studies in the adult stage of this line are warranted. Especially, the knock-out model might require appropriate challenging, or the strain should be crossed with other genetically engineered mouse models with cancer or immune deficiency-related phenotypes. Note that, so far, studies in cancer have been done with a single transplanted tumor model: B16 melanoma cells (see Fazzari et al., 2007). The immunological phenotype has not been studied in these mice so far.

Heterozygous:
The heterozygous mice do not show any phenotype.
Breeding historyChimeric mice were initially generated on 129 x C57BL/6 background, and then subjected to several generations of backcrossing. The strain is commonly bred in homozygous state.
References
  • Plexin-B1 plays a redundant role during mouse development and in tumour angiogenesis.;Fazzari Pietro, Penachioni Junia, Gianola Sara, Rossi Ferdinando, Eickholt Britta J, Maina Flavio, Alexopoulou Lena, Sottile Antonino, Comoglio Paolo Maria, Flavell Richard A, Tamagnone Luca, ;2007;BMC developmental biology;7;55; 17519029
  • Sema4C-Plexin B2 signalling modulates ureteric branching in developing kidney.;Perälä Nina, Jakobson Madis, Ola Roxana, Fazzari Pietro, Penachioni Junia Y, Nymark Mariann, Tanninen Tiina, Immonen Tiina, Tamagnone Luca, Sariola Hannu, ;2011;Differentiation; research in biological diversity;81;81-91; 21035938
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

  • Plexin-B1 plays a redundant role during mouse development and in tumour angiogenesis.;Fazzari Pietro, Penachioni Junia, Gianola Sara, Rossi Ferdinando, Eickholt Britta J, Maina Flavio, Alexopoulou Lena, Sottile Antonino, Comoglio Paolo Maria, Flavell Richard A, Tamagnone Luca, ;2007;BMC developmental biology;7;55; 17519029
  • Sema4C-Plexin B2 signalling modulates ureteric branching in developing kidney.;Perälä Nina, Jakobson Madis, Ola Roxana, Fazzari Pietro, Penachioni Junia Y, Nymark Mariann, Tanninen Tiina, Immonen Tiina, Tamagnone Luca, Sariola Hannu, ;2011;Differentiation; research in biological diversity;81;81-91; 21035938

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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